Effect of Pamidronate on Protein Adsorption and Osteoblast Adhesion to Hydroxyapatite Bioceramics Scaffold
This study is to investigate the effect of PAM on protein adsorption and osteoblastic cells adhesion to HA bioceramics. PAM was immobilized on the surface of HA bioceramics for bone scaffold by chelating. The outermost layer of the specimens was analyzed by XPS and FT-IR. The protein adsorption test was performed using the 10% bovine calf serum absorbed on the specimens in vitro. The osteoblastic cells were inoculated and cultured on the scaffolds. SEM, MTT test and ALP activity test evaluated the cell attachment, proliferation and activity on the scaffolds. Characteristic peaks in XPS and FT-IR spectra indicated PAM being immobilized on the surface of the bioceramics. PAGE and 2-D DIGE results indicated that HA absorbed more acidic proteins, while PAM-HA absorbed more basic and neutral proteins.The cell culture test indicated that the cells actively proliferated on the scaffolds. There was no significant difference between the ALP activity of the cells cultured for 1d, 3d, 5d and 7d on PAM-HA and that of the controls. PAM had no obvious effect on the cytocompatibility of HA, and PAM-HA bioceramics could be used as bone scaffold with potential ability to improve osteogenesis.
Xingdong Zhang, Xudong Li, Hongsong Fan, Xuanyong Liu
E. Luo et al., "Effect of Pamidronate on Protein Adsorption and Osteoblast Adhesion to Hydroxyapatite Bioceramics Scaffold", Key Engineering Materials, Vols. 330-332, pp. 885-888, 2007