Biosensor Application of Enzyme-Functionalized Titania/Titanium Composite
Highly-ordered titania nanotube array has been fabricated by a potentiostatic anodization process in acidic fluoride electrolyte. Calcination at high temperature is followed to achieve crystallization from amorphous to anatase phase. The approach of embedding bioactive enzymes inside titania tubule channels has been applied for bioactivation modification of titania/titanium. The electropolymerization process is applied by using conductive pyrrole monomer to achieve surface immobilization of enzymes to improve interfacial connection. Characterizations including field emission scanning electron microscopy, nano-indentation and linear sweep voltammetry measurements have been performed to fully evaluate the surface morphology, nanomechanical and electrochemical properties of this composite. Molecule detection application has been investigated by applying such an enzyme-titania/titanium composite.
J.K. Kim, D.Z. Wo, L.M. Zhou, H.T. Huang, K.T. Lau and M. Wang
Y. B. Xie et al., "Biosensor Application of Enzyme-Functionalized Titania/Titanium Composite", Key Engineering Materials, Vols. 334-335, pp. 645-648, 2007