Authors: Seung Jae Lee, Byung Kim, Jin Sang Lee, Sung Won Kim, Min Soo Kim, Joo Sung Kim, Geun Bae Lim, Dong Woo Cho
Abstract: Understanding chondrocyte behavior inside complex, three-dimensional environments
with controlled patterning of geometrical factors would provide significant insights into the basic
biology of tissue regenerations. One of the fundamental limitations in studying such behavior has
been the inability to fabricate controlled 3D structures. To overcome this problem, we have
developed a three-dimensional microfabrication system. This system allows fabrication of
predesigned internal architectures and pore size by stacking up the photopolymerized materials.
Photopolymer SL5180 was used as the 3D microfabricated scaffolds. The results demonstrate that
controllable and reproducible inner-architecture can be fabricated. Chondrocytes from human nasal
septum were cultured in 3D scaffolds for cell adhesion behavior. Such 3D scaffolds might provide
effective key factors to study cell behavior in complex environments and could eventually lead to
optimum design of scaffolds in various tissue regenerations such as cartilage, bone, etc. in a near
future.
723
Authors: Jin Sang Lee, Byung Kim, Min Soo Kim, Seung Jae Lee, Sung Won Kim, Dong Woo Cho, Joo Sung Kim, Geun Bae Lim
Abstract: In this study, we investigated the effect of the use of alginate sponge as a chondrocyte-3D
scaffold for the construction of a cartilage graft. Alginate sponge was made by 5% alginic acid
which was crosslinked by CaCl2. Chondrocytes were obtained from a nasal septum after the
operation and cultured in 3D alginate sponge. For analysis of cell differentiation, we have checked
aggrecan, collagen type I and II using RT-PCR and performed the histological and scanning
electron microscopy analysis. Our experiments showed that alginate sponge of 5% promoted
sufficient chondrocyte proliferation and differentiation, resulting in the formation of a specific
cartilage matrix. The sponge presents new perspectives with respect to in vitro production of
"artificial" cartilage. We conclude that the alginate sponges have potential as a scaffold for cartilage
tissue engineering.
883
Authors: H. Lu, S.M. Zhang, L. Cheng, P.P. Chen, W. Zhou, Jian Liu, J.X. Zhou
Abstract: A novel porous composite scaffold of nano-HA/poly (lactic-co-glycolic) (PLGA) was
fabricated by solvent casting/particulate leaching method. Chondrocytes were isolated from the
knee articular joints of a rabbit, and then seeded in the scaffolds. The cell-loaded scaffolds were
cultured in vitro for 5 days before implantation. Full-thickness articular cartilage defects were
created in rabbits, and filled with and without the cell-loaded nano-HA/PLGA scaffolds. The
implants were harvested after in vivo incubation of 2 and 5 weeks. Cartilaginous tissues were
observed at defects repaired with the cell-loaded scaffolds, while only fibrous tissues were found
for the control groups. The repaired tissues were evaluated histologically by hematoxylin and eosin
staining. Results revealed that nano-HA/PLGA composite scaffolds facilitated adheration of cells in
vitro, and the nano-HA particles could prevented the scaffolds from collapsing and promoted the
formation of cartilaginous tissue in vivo.
1185
Authors: Ri Long Jin, So Ra Park, Jeong Hwa Son, Byoung Hyun Min
Abstract: Two passaged (P2) immature porcine articular chondrocytes were used to fabricate an
engineered cartilage tissue in an in vitro scaffold-free system with or without insulin like growth
factor 1 (IGF-1). This study shows the possibility of the fabrication of structurally regular neocartilage
tissue using passaged chondrocytes in the scaffold-free system with insulin like growth
factor-1(IGF-1).
149
Authors: J. Sun, R. Wang, L. Zheng, Yan Fei Tan, Yu Mei Xiao, Hong Song Fan, Xing Dong Zhang
Abstract: With good biocompatibility, collagen is often used in cartilage tissue engineering. Collagen/alginate composite was hoped to improve the poor mechanical property of pure collagen but the biocompatibity was decreased. In this study, hydroxyapatite (HA) particles were used to get collagen/alginate/HA (CAHA) composite film to enhance the bioactivity properties. The bioactivity of the composite was investigated by in vitro co-culture with chondrocytes. During the 6-day cell culture in vitro, the composite showed a significant improvement in promoting proliferation and maintaining morphology/phenotype of the chondrocytes over collagen/alginate composite by MTT, SEM, fluorescent and immunohistochemical assays. Cytocompatibility and cytoviablility of CAHA even come up to that of collagen film alone. The results indicated that the composite film may provide an appropriate environment for the proliferation and maintaining the morphology and phenotype of chondrocytes and have a potential clinical application in the cartilage tissue engineering field.
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