Bisphosphonate Pamidronate Modifying Hydroxyapatite Bioceramics for Hard Tissue Repair
This study is to investigate the method of pamidronate modifying HA bioceramics and the effect of pamidronate modifying HA bioceramics on the osteoblastic cells. Pamidronate was immobilized on the surface of porous HA bioceramics for bone scaffold by chelating. The outermost layer of the specimens was analyzed by XPS and FT-IR. The depth profile was investigated by the argon-ion sputtering method. The MC3T3-E1 osteoblastic cells were inoculated and cultured on the scaffolds. SEM and phase contrast microscope observation of the cells, MTT test and ALP activity test evaluated the cell attachment, proliferation and activity on the scaffolds. Characteristic peaks in XPS and FT-IR spectra indicated pamidronate being immobilized on the surface of the bioceramics. The cell culture test indicated that the cells actively proliferated on the scaffolds. There was no significant difference between the ALP activity of the cells cultured for 1d, 3d and 7d on PAM-HA and that of the controls. The result indicated that PAM-HA bioceramics had favorable cytocompatibility to be used as bone scaffold with potential ability to improve osteogenesis.
Wei Pan and Jianghong Gong
E. Luo et al., "Bisphosphonate Pamidronate Modifying Hydroxyapatite Bioceramics for Hard Tissue Repair", Key Engineering Materials, Vols. 336-338, pp. 1711-1714, 2007