A Novel Micro-Dissection Method Using Ultrasonic Vibration for Molecular Analysis


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Molecular techniques are transforming our understanding of cellular function and disease. However, accurate molecular analysis methods will be limited if the input DNA, RNA, or protein is not derived from pure population of cells or is contaminated by the wrong cells. A novel Ultrasonic Vibration Microdissection (UVM) method was proposed to procure pure population of targeted cells from tissue sections for subsequent analysis. The principle of the Ultrasonic Vibration Cutting is analyzed, and a novel microknife is designed. A multilayer piezoelectric actuator is used to actuate a sharp needle vibrating with high frequency and low amplitude (Approx. 16–50 kHz, and 0-3μm) to cut the tissue. Contrast experiment was done to test the feasibility of UVM method. Experimental results show that the embedded tissue can be quickly and precisely cut with the ultrasonic vibration micro-dissection method.



Edited by:

Shen Dong and Yingxue Yao




L.G. Chen et al., "A Novel Micro-Dissection Method Using Ultrasonic Vibration for Molecular Analysis", Key Engineering Materials, Vol. 339, pp. 291-296, 2007

Online since:

May 2007




[1] Fumihito Arai, Talaharu Amano and Toshio Hltuda: IEEE International Symposium on Micromechatronics and Human Science, (2000), p.195~200.

[2] Fumihito Arai, Talaharu Amano and Toshio Hltuda: IEEE International Conference on Robotics 8 Automation. Seoul, Korea, May 21-26, p.139~145.

[3] C.X. Ma, E. Shamoto, T. Moriwaki and L.J. Wang: International Journal of Machine Tools & Manufacture, Vol. 44 (2004), p.1305~1310.

[4] Shu Karube, Wataru Hoshino, Tatsuo Soutome and Keijin Sato: International Journal of Non-Linear Mechanics, Vol. 37 (2002), p.541~564.

DOI: https://doi.org/10.1016/s0020-7462(01)00031-2

[5] Solomon Max: Direct AWG sine wave synthesis with fixed clock frequency Measurement, Vol. 31 (2002), p.20~217.

DOI: https://doi.org/10.1016/s0263-2241(01)00027-6

[6] Karin Schutze, Ingrid Becker and et al: Genetic Analysis: Biomolecular Engineering, Vol. 14 (1997), p.1~8.

[7] J.B. Helen, M.P. Brent and D. Catherine: Isolating vessels from the mouse brain for gene expression analysis using laser capture microdissection Brain Research Protocols, Vol. 9 (2002), pp.206-213.

DOI: https://doi.org/10.1016/s1385-299x(02)00147-2

[8] F. Arar and K. Morishima: Bio-micro-manipulation. Proc of IEEE International Conference on IROS (1997), pp.1300-130.