A Developmental Study of Artificial Skin Using the Alginate Dermal Substrate
Alginate, which is isolate from brown seaweed, a bioabsorbable long chain polysaccharides of guluronic acid and mannuronic acid. The authors produced alginate, fibroblastgrowth- factor mixed alginate and alginate-collagen complex as a disc form. For study of wound healing, full thickness skin defects were made on the dorsal area of the animal model. And then alginate, fibroblast-growth-factor mixed alginate, alginate-collagen complex, vaseline gauze as control were applied on the wound and evaluated grossly and histopathologically. For biocompatibility test, alginate and alginate-collagen complex discs were implanted on back of New Zealand white rabbits. And we measured size of wound healing, size of wound epithelization, size of wound contracture at 5, 10, 15, 20, and 25 days. Four weeks after implantation, implanted animals were examined immunologically against alginate and collagen. Alginate and FGF-mixed alginate, alginate-collagen complex group showed statistically higher percentage of wound contraction and wound healed than control group(p<0.05) Alginate-collagen complex group and FGF-mixed alginate group showed statistically higher percentage of wound healed than alginate group. In conclusion, high-purified alginate from seaweeds by our purification method, has the ability of wound healing and addition of FGF or collagen increase the ability of alginate, it shows the possibility of alginate as dermal substrate.
Young-Ha Kim, Chong-Su Cho, Inn-Kyu Kang, Suk Young Kim and Oh Hyeong Kwon
D. H. Park et al., "A Developmental Study of Artificial Skin Using the Alginate Dermal Substrate", Key Engineering Materials, Vols. 342-343, pp. 125-128, 2007