Injectable Chitosan Carrier for Demineralized Bone Matrix


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In this study, we developed an injectable carrier for demineralized bone matrix (DBM) which consists of chitosan and glycerol phosphate (GP). The phase transition behaviors of chitosan and GP solution characterized as a function of temperature. The chitosan and GP solutions were shown to form a sol at room temperature, and the chitosan solution with 10-20 wt% concentrations were found to undergo sol-to-gel phase transitions as the temperature was increased. In situ gel forming implant in vivo was successfully fabricated by simple subcutaneous injection of chitosan and GP solutions with demineralized bone matrix (DBM). von Kossa images of the gel implant formed from solution with DBM revealed the presence of mineral deposits.



Key Engineering Materials (Volumes 342-343)

Edited by:

Young-Ha Kim, Chong-Su Cho, Inn-Kyu Kang, Suk Young Kim and Oh Hyeong Kwon




Y. H. Kim et al., "Injectable Chitosan Carrier for Demineralized Bone Matrix", Key Engineering Materials, Vols. 342-343, pp. 177-180, 2007

Online since:

July 2007




[1] R.W. Bucholz: Clin. Orthop. Relat. Res. Vol. 395 (2002), p.44.

[2] S.R. Park, B.H. Min, S.H. Park and H.J. Lee: Tissue Eng. Regen. Medicine Vol. 2 (2005), p.77.

[3] P.J. Boyne: Bone Vol. 19 (1996) , S83-S92.

[4] A. Gutowska, B. Jeong and M. Jasionowski: Anat. Rec. Vol. 263 (2001), p.342.

[5] K.S. Seo, K.D. Hong, H. Hyun, M.S. Kim, G. Khang and H.B. Lee: Tissue Eng. Regen. Med. Vol. 2 (2005), p.109.

[6] M.S. Kim, H. Hyun, G. Khang and H.B. Lee: Macromolecules Vol. 39 (2006), p.3099.

[7] M.S. Kim, H. Hyun, K.S. Seo, Y.H. Cho, G. Khang and H.B. Lee: J. Polym. Sci. Part A: Polym. Chem. Vol. 44 (2006), p.5413.

[8] B.D. Ratner, A.S. Hoffman, F.J. Schoen and J.E. Lemons, Biomaterials science: An introduction to materials in medicine. 2 nd Ed. New York: Academic Press, (2004).

[9] A. Chenite, M. Buschmann, D. Wang and C. Chaput, N. Kandani, Carbohydrate Polymers Vol. 46 (2001), p.39.

[10] A.H. Reddi and W.A. Anderson: J. Cell. Bio. Vol. 69 (1976), p.557.