Biodegradable poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nanofibrous matrix containing gelatin was fabricated by electrospinning method. The average diameter of electrospun PHBV/Gelatin (1:1) nanofibers was 600 nm determined by FE-SEM. ATR-FTIR and ESCA measurements were used to confirm the presence of gelatin in PHBV/Gelatin nanofibers. Human fibroblasts' behavior on PHBV/Gelatin nanofibrous matrix has been investigated. Fibroblasts were well attached on the surface of control PHBV and PHBV/Gelatin nanofibers. Initial cell attachment on PHBV/Gelatin nanofibers was higher than that of control PHBV nanofibers. Gelatin has many RGD moiety that mediate cell attachment. From this reason, initial cell attachment increased on the surface of PHBV/Gelatin nanofibers. From the results, coelectrospinning of PHBV and gelatin is a promising method for tissue engineering scaffold.