In Vitro Chondrocyte Culture Using Hydrophilized PLLA Scaffold in Bioreactor System


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Nonporous PLLA film and porous PLLA scaffolds were prepared and then grafted with acrylic acid (AA) using in situ direct plasma treatment to obtain PLLA-g-PAA. Chondrocytes isolated from rabbit knee articular cartilages were cultivated in Dulbecco’s modified eagle medium- F12 (DMEM-F12) containing 10% fetal bovine serum (FBS) and 1% antibiotics and passaged twice before cell seeding. Once seeded on either PLLA films or scaffolds, they were placed in a bioreactor system and an intermittent hydrodynamic pressure (IHP) was applied in 3 bars, while turned on for 2 min and off for 28 min during 15-day culture. AA grafting to PLLA surface was confirmed from various surface analyses. From WST-1 assay, chondrocyte proliferation was significantly improved with dynamic IHP for PLLA and PLLA-g-PAA scaffolds as compared to static culture. This study indicates that IHP may have significant influence on chondrocytes behavior in 3D culture environment.



Key Engineering Materials (Volumes 342-343)

Edited by:

Young-Ha Kim, Chong-Su Cho, Inn-Kyu Kang, Suk Young Kim and Oh Hyeong Kwon




H. S. Yang et al., "In Vitro Chondrocyte Culture Using Hydrophilized PLLA Scaffold in Bioreactor System", Key Engineering Materials, Vols. 342-343, pp. 253-256, 2007

Online since:

July 2007




[1] Y. B. Bouchet, M. Colon, A. Polotsky, A. H. Shikani, D. S. Hungerford, C. G. Frondoza: J. Biomed. Mater. Res. Vol 52 (2000), p.716.

[2] H. C. Chen, H. -P. Lee, Y. -C. Ho, M. -L. Sung, Y. -C. Hu: Biomaterials Vol 27 (2006), p.3154.

[3] L. E. Freed, A. P. Hollander, I. Martin, J. R. Barry, R. Langer, and G. Vuniak-Novakovic: Exp. Cell. Res. Vol. 240 (1998), p.58.

[4] T. Ikenoue. T. C. D. Trindade, M. S. Lee, E. Y. Lin, D. J. Schurman, S. B. Goodman, R. L. Smith: J. Orth. Res. Vol 21 (2003), p.110.

[5] U. Hansen, M. Schunke, C. Domm, N. Ioannidis, J. Hassenpflug, T. Gehrke, B. Kurz: J. Biomechanics Vol 34 (2001), p.941 �.

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