Effect of Collagen Coating on Lipid Accumulation and Differentiation on 3T3-L1 Preadipocyte Treated with Conjugated Linoleic Acid


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The purpose of this study is to make use of trans10,cis12 CLA (t-CLA) that has potential for proliferation and differentiation to form adipocyte on the collagen-coated surface. Results provided evidences of good adhesion, growth, viability, and differentiation of adipocyte on collagen-coated surface compared with non-coated surface. Also, the results showed that mouse 3T3-L1 preadipocyte can be successfully and reproducibly cultured on the collagen-coated surface, and the adipocyte precursor cells placed on the collagen-coated surface are able to undergo full maturation into adipocytes in the control cells. Moreover, glycerol-3-phosphate dehydrogenase (GPDH) activity in 3T3-L1 preadipocyte cultured on collagen-coated surface with t-CLA was higher than that on polystyrene (PS) surface due to higher cell adhesion and cell viability. These results suggest that collagen coating may provide a promising approach to develop a new adipocyte replacement strategy using CLA.



Key Engineering Materials (Volumes 342-343)

Edited by:

Young-Ha Kim, Chong-Su Cho, Inn-Kyu Kang, Suk Young Kim and Oh Hyeong Kwon




H. S. Moon et al., "Effect of Collagen Coating on Lipid Accumulation and Differentiation on 3T3-L1 Preadipocyte Treated with Conjugated Linoleic Acid", Key Engineering Materials, Vols. 342-343, pp. 261-264, 2007

Online since:

July 2007




[1] H. K. Kleinman, M. L. McGarvey, J. R. Hassell, V. L. Star, F. B. Cannon, G. W. Laurie, G. R. Martin. Biochemistry 25 (1986) 312-318.

[2] R. O. Hynes. (2002) Nat. Med. 8, 918 - 921.

[3] G. J. Hausman, J. T. Wright, R. L. Richardson. J. Anim. Sci. 74 (1996) 2117-2128.

[4] Y. Park, K. J. Albright, W. Liu, J. M. Storlson, M. E. Cook, M. W. Pariza. Lipids 32 (1997) 853858.

[5] Y. Choi, Y. Kim, Y. Han, Y. Park, M. W. Pariza. J. Nutr. 130 (1999) 1920-(1924).

[6] L. S. Wise, H. Green. J. Biol. Chem. 254 (1979) 273-275.

[7] M. M. Bradford. Anal Biochem. 72 (1976) 248-254.

[8] K. N. Lee, M. W. Pariza. Biochem. Biophys. Res. Commun. 248 (1998) 817-821.

[9] R. L. Mcneel, E. O. Smith, H. J. Mersmann. In Vitro Cell Dev. Biol. Anim. 39 (2003) 375-382.

[10] A. E. Brodie, V. A. Manning, K. R. Ferguson, D. E. Jewell, C. Y. Hu. J. Nut. 129 (1999) 602606.

[11] S. H. Hsu, C. Y. Chen, P. S. Lu, C. S. Lai, C. J. Chen. Biotechnol. Bioeng. 92 (2005) 579-588.

[12] A. Yuda, S. Ban, Y. Izumi. Dent. Mater J. 24 (2005) 588-595.

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