Evaluation of RGD-Grafted Porous PLLA Scaffold for Chondrogenic Differentiation of Stem Cells

Abstract:

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Biodegradable porous poly(L-lactic acid) (PLLA) scaffolds were prepared using gas foaming method. The PLLA scaffolds with a hydrophobic surface were subjected to Ar plasma treatment and in situ acrylic acid (AA) grafting to obtain hydrophilic PLLA scaffold (PLLA-PAA). Cell-adhesive RGD peptide was then immobilized onto the AA-grafted PLLA (PLLA-PAA-RGD). Once rabbit bone marrow-derived mesenchymal stem cells (BM-MSC) were isolated, MSCs were seeded into PLLA control, PLLA-PAA, and PLLA-PAA-RGD scaffold and cultured for up to 4 weeks in chondrogenic medium with the addition of 10 ng/ml transforming growth factor (TGF)-β1. Surface analysis of AA-grafted PLLA identified significant alterations of surface characteristics, including reduced contact angle and different atomic compositions. From WST-1 assay at 4 weeks, cells were found more proliferative in PLLA-PAA than the others. Upon the histological analysis of Safranin O staining, chondrogenic differentiation of MSCs appeared to be progressed more actively in PLLA-PAA. The effect of RGD immobilization on MSC differentiation was barely notable.

Info:

Periodical:

Key Engineering Materials (Volumes 342-343)

Edited by:

Young-Ha Kim, Chong-Su Cho, Inn-Kyu Kang, Suk Young Kim and Oh Hyeong Kwon

Pages:

297-300

DOI:

10.4028/www.scientific.net/KEM.342-343.297

Citation:

H. J. Jung et al., "Evaluation of RGD-Grafted Porous PLLA Scaffold for Chondrogenic Differentiation of Stem Cells", Key Engineering Materials, Vols. 342-343, pp. 297-300, 2007

Online since:

July 2007

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Price:

$35.00

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