Thermosensitive and Cell-Adhesive Pluronic Hydrogels for Human Adipose-Derived Stem Cells

Abstract:

Article Preview

Pluronic F127 has received increasing attention over many years as drug delivery systems, biomaterials, and hydrogels for tissue engineering. In this study, we synthesized temperature-sensitive and cell-adhesive triblock F127 copolymers, in which Arg-Gly-Asp (RGD) peptide ligand was grafted to Pluronic F127-4-methacryloxyethyl trimellitic anhydride (4-META) to obtain F127-META-RGD. The chemical structures of the F127-META-RGD block copolymers were confirmed by FTIR, 1H and 13C NMR, and GPC. The resultant F127-META-RGD showed very similar thermosensitive behaviors to F127 and F127-META. The critical micelle temperature (CMT) of the F127 copolymers decreased in the order of F127 < F127-META < F127-META-RGD, whereas the particle size followed an opposite trend. Interactions between the F127 copolymers and adipose-derived stem cells (ASC) were evaluated in terms of cell adhesion and proliferation on the hydrogel. These thermosensitive RGD-grafted Pluronic hydrogels that display the enhanced cell adhesiveness, are expected to be useful as a functional injectable scaffold for tissue engineering.

Info:

Periodical:

Key Engineering Materials (Volumes 342-343)

Edited by:

Young-Ha Kim, Chong-Su Cho, Inn-Kyu Kang, Suk Young Kim and Oh Hyeong Kwon

Pages:

301-304

Citation:

K. D. Park et al., "Thermosensitive and Cell-Adhesive Pluronic Hydrogels for Human Adipose-Derived Stem Cells", Key Engineering Materials, Vols. 342-343, pp. 301-304, 2007

Online since:

July 2007

Export:

Price:

$38.00

[1] Y.L. Cao, A. Rodriguez, M. Vacanti, C. Ibarra, C. Arevalo, and C.A. Vacanti; J Biomater Sci Polym Ed Vol. 9 (1998), p.475.

[2] S. Terada, H. Yoshimoto, J.R. Fuchs, M. Sato, I. Pomerantseva, M.K. Selig, D. Hannouche, and J.P. Vacanti, J Biomed Mater Res Vol. 74A (2005), p.907.

DOI: https://doi.org/10.1002/jbm.a.30505

[3] Medical Functional Materials, ed. T. Tsuruda, Japan Polymer Society, Public Publication (1990).

[4] U. Hersel, C. Dahmen, and H. Kessler; Biomaterials Vol. 24 (2003), p.4385.

[5] H.S. Shin, S.B. Jo, and A.G. Mikos; Biomaterials Vol. 24 (2003), p.4353.

[6] H.A. Awad, M.Q. Wickham, H.A. Leddy, J.M. Gimble, and F. Guilak; Biomaterials Vol. 25 (2004), p.3211.

[7] S.Y. Park, B.R. Han, K.M. Na, D.K. Han, and S.C. Kim; Macromolecules Vol. 36 (2003), p.4115.

Fetching data from Crossref.
This may take some time to load.