Thermosensitive and Cell-Adhesive Pluronic Hydrogels for Human Adipose-Derived Stem Cells

Abstract:

Article Preview

Pluronic F127 has received increasing attention over many years as drug delivery systems, biomaterials, and hydrogels for tissue engineering. In this study, we synthesized temperature-sensitive and cell-adhesive triblock F127 copolymers, in which Arg-Gly-Asp (RGD) peptide ligand was grafted to Pluronic F127-4-methacryloxyethyl trimellitic anhydride (4-META) to obtain F127-META-RGD. The chemical structures of the F127-META-RGD block copolymers were confirmed by FTIR, 1H and 13C NMR, and GPC. The resultant F127-META-RGD showed very similar thermosensitive behaviors to F127 and F127-META. The critical micelle temperature (CMT) of the F127 copolymers decreased in the order of F127 < F127-META < F127-META-RGD, whereas the particle size followed an opposite trend. Interactions between the F127 copolymers and adipose-derived stem cells (ASC) were evaluated in terms of cell adhesion and proliferation on the hydrogel. These thermosensitive RGD-grafted Pluronic hydrogels that display the enhanced cell adhesiveness, are expected to be useful as a functional injectable scaffold for tissue engineering.

Info:

Periodical:

Key Engineering Materials (Volumes 342-343)

Edited by:

Young-Ha Kim, Chong-Su Cho, Inn-Kyu Kang, Suk Young Kim and Oh Hyeong Kwon

Pages:

301-304

DOI:

10.4028/www.scientific.net/KEM.342-343.301

Citation:

K. D. Park et al., "Thermosensitive and Cell-Adhesive Pluronic Hydrogels for Human Adipose-Derived Stem Cells", Key Engineering Materials, Vols. 342-343, pp. 301-304, 2007

Online since:

July 2007

Export:

Price:

$35.00

In order to see related information, you need to Login.

In order to see related information, you need to Login.