Effect of Poly (Lactic–Co–Glycolic Acid) (75:25)/ Hydroxyapatite Composite on Chondrocyte Culture for Tissue Reconstruction


Article Preview

PLGA (75:25)/hydroxyapatite (HA) composite films were fabricated by solvent-casting method to investigate the effect of various hydroxyapatite content ratio to the PLGA film for cellular attachment and proliferation. Mechanical property of the composite film was characterized by tensile test. The ultimate tensile strength of 10% HA content film was two folds higher than control group. The surface of the film was characterized by contact angle measurement. The PLGA/HA composite film was more hydrophilic than control film. In vitro chondrocyte responses to the composite films were measured by cellular attachment and proliferation test. The attached and proliferated cells were significantly higher on PLGA/HA (10%) composite film than control group (1.44 times higher in attachment test and 1.31 times higher for 6th-day at culture in proliferation assaying, p<0.05). Base on these finding, the PLGA/HA (10%) composite was effective for the cell attachment for the initial stage of cultivation and cell proliferation.



Key Engineering Materials (Volumes 342-343)

Edited by:

Young-Ha Kim, Chong-Su Cho, Inn-Kyu Kang, Suk Young Kim and Oh Hyeong Kwon




J. B. Lee et al., "Effect of Poly (Lactic–Co–Glycolic Acid) (75:25)/ Hydroxyapatite Composite on Chondrocyte Culture for Tissue Reconstruction ", Key Engineering Materials, Vols. 342-343, pp. 353-356, 2007

Online since:

July 2007




[1] Hunziker EB: Osteoarthritis Cartilage Vol. 10 (2002), p.423.

[2] A. Sinha, G. Das, B. K. Sharma, R. P. Roy, A. K. Pramanick, S. Nayar : Mater Sci and Eng (2006).

[3] Kikuchi M, Suetsugu Y, Tanaka J, Akao M. : J Mater Sci Mater Med Vol. 8(1997), p.361.

[4] Z. Hong, P. Zhang, C. He, X. Qiu, A. Liu, L. Chen, X. Chen, X. Jing: Biomaterials Vol. 26 (2005), p.6296.

[5] C. Durucan, P. W. Brown.: Journal of Biomedical Materials Research Vol. 51 (2000), 717-725.

[6] Sarah E. Petricca, Kacey G. Marra, Prashant N. Kumta. : Acta Biomaterialia Vol. 2(2006), p.277.

[7] Sang-Soo Kim, Min Sun Park, Oju Jeon, Cha Yong Choi: Biomaterials Vol. 27(2006), p.1859.

DOI: https://doi.org/10.1016/j.biomaterials.2005.08.016

[8] Carnagey J, Anderson DH, Ranieri J : J Biomed Mater Res Vol. 65B (2003), p.171.

[9] Buschmann MD, Gluzband YA, Grodzinsky AJ et al. : J Cell Sci Vol. 108 (1995), p.1497.

[10] Zhongkui Hong, Peibiao Zhang, Chaoliang He, Xueyu Qiu, Aixue Liu, Li Chen, Xuesi Chen, Xiabin Jing : Biomaterials Vol. 26 (2005).