The aim of this study was to evaluate the regenerative effects of chitosan membranes containing tetracycline (TC) applied to surgically created one-wall intrabony defects in beagle dogs. The defects either received chitosan membrane (CH), chitosan membrane containing 0.5%, 1% TC (CH-TC0.5, CH-TC1.0) or flap operation only. The animals were sacrificed 8 weeks after the experimental surgery, and comparative histological and histometric examinations were done. The amount of junctional epithelium migration and connective tissue adhesion did not show any statistically significant differences among the groups. The amount of new cementum regeneration, and new bone regeneration were significantly greater in CH-TC1.0 group than the control (P<0.05). The results suggest that chitosan membrane combined with 1.0% TC may have beneficial effect on the regeneration of bone and cementum in intrabony periodontal defects.