The effect of β-glucan-reinforced PLGA scaffold on cell proliferation was investigated. The PLGA scaffolds were prepared by salt-leaching method. The prepared scaffolds were grafted with (1→3) (1→6)-β-glucan in various ratios after plasma treatment on the surface. The surface of the scaffold was characterized by scanning electron microscope (SEM). The HDFs (Human dermal fibroblasts, 1105 cells/scaffold) were used to evaluate the cell proliferation on PLGA scaffold before and after plasma/β-glucan treatment. In results, in the β-glucan treated scaffolds, the pores seemed to become narrower and even looked like closed form. The result of cell proliferation showed that the plasma/β-glucan treated scaffolds had narrower pores because the β-glucan was attached in the pores that would not be allowed the cells to penetrate into the inner areas. Consequently, cell proliferation was not effective in the plasma/β-glucan treated scaffolds in this study.