Effect of (1→3)(1→6)-Β-Glucan Containing PLGA Scaffold on Human Dermal Fibroblast Attachment and Proliferation


Article Preview

The effect of β-glucan-reinforced PLGA scaffold on cell proliferation was investigated. The PLGA scaffolds were prepared by salt-leaching method. The prepared scaffolds were grafted with (1→3) (1→6)-β-glucan in various ratios after plasma treatment on the surface. The surface of the scaffold was characterized by scanning electron microscope (SEM). The HDFs (Human dermal fibroblasts, 1105 cells/scaffold) were used to evaluate the cell proliferation on PLGA scaffold before and after plasma/β-glucan treatment. In results, in the β-glucan treated scaffolds, the pores seemed to become narrower and even looked like closed form. The result of cell proliferation showed that the plasma/β-glucan treated scaffolds had narrower pores because the β-glucan was attached in the pores that would not be allowed the cells to penetrate into the inner areas. Consequently, cell proliferation was not effective in the plasma/β-glucan treated scaffolds in this study.



Key Engineering Materials (Volumes 342-343)

Edited by:

Young-Ha Kim, Chong-Su Cho, Inn-Kyu Kang, Suk Young Kim and Oh Hyeong Kwon




S. G. Lee et al., "Effect of (1→3)(1→6)-Β-Glucan Containing PLGA Scaffold on Human Dermal Fibroblast Attachment and Proliferation", Key Engineering Materials, Vols. 342-343, pp. 377-380, 2007

Online since:

July 2007




[1] Antia W. T Shum, Jiashen Li, Arthur F. T mak: Poly Deg stab 87 (2005), p.487.

[2] M. Ramchandani, D. Robinson: J Controlled Release 54 (1998), p.167.

[3] Gao J, Niklason L, Langer R: J Biomed Mater Res 42 (1999), p.417.

[4] J. Yang, J Bei, S. Wang: Biomaterials 23 (2002), p.2607.

[5] JJ. Yoon, SN. Nam, JH. Kim, TG. Park: Inc. Biotechnol Bioeng 78 (2002), p.1.

[6] SG. Lee, EY. An, JB. Lee, JC. Park, JW. Shin, JK. Kim: Sur and Coat Tech (2006) Accepted.

[7] HJ. Son, HC. Bae, HJ. Kim, DH. Lee, DW. Han, JC. Park: Current App Phy 5 (2005), p.468.