A Tissue Engineering Based Approach to Regeneration of Intervertebral Disc


Article Preview

Thermosensitive scaffold was suggested for the partial replacement of nucleus in degenerated intervertebral disc with a minimally invasive surgical procedure. Biocompatible and thermosensitive scaffolds were prepared by coupling reaction of Pluronic with chitosan and it was inserted in degenerative spinal motion segments. To confirm the regeneration of degenerative discs and subsequent structural stability, cell proliferation and morphological changes were evaluated using relaxation time, quantity of DNA and histological examination. As a result, inserted group showed higher relaxation time, reduced the decrement of DNA contents, and accumulated GAG amount. The results confirmed the potential of thermosensitive scaffolds and minimally invasive surgical procedure for the regeneration of degenerative discs.



Key Engineering Materials (Volumes 342-343)

Edited by:

Young-Ha Kim, Chong-Su Cho, Inn-Kyu Kang, Suk Young Kim and Oh Hyeong Kwon




Y.J. Lee et al., "A Tissue Engineering Based Approach to Regeneration of Intervertebral Disc", Key Engineering Materials, Vols. 342-343, pp. 397-400, 2007

Online since:

July 2007




[1] BH Guiot, RG Fessler. Neurosurgery, Vol. 47 (2000), p.1034.

[2] MA Di, AR Vaccaro, JY Lee, V Denaro, MR Lim, Spine, Vol. 30 (2005), p.16.

[3] J Thomas, A Lowman, M Marcolongo. J Biomed Mater Res A, Vol. 67 (2003), p.1329.

[4] MA Wiseman, HL Birch, M Akmal, AE Goodship, Spine, Vol. 30 (2005), pp.505-511.

[5] M Okuma, J Mochida, K Nishimura, K Sakabe, K Seiki, J Orthop Res, Vol. 18 (2000), p.988.

[6] H Ishihara, K Warensjo, S Roberts, JP Urban, Am J Physiol, Vol. 272 (1997), p.1499.

[7] C Park, YJ Kim, CS Lee, K An, HJ Shin, CH Lee, CH Kim, JW Shin, Spine, Vol. 30 (2005).