Cartilage Tissue Engineering using a Elastic Poly (L-Lactide-co-ε-Caprolactone) Scaffold

Abstract:

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Mechano-active scaffolds were fabricated from very elastic poly(L-lactide-co-ε-carprolactone). The scaffolds with 80 % porosity and 300~500 μm pore size were prepared by a gel-pressing method. As a control group for elastic properties of polymer scaffolds, rigid poly L-lactide scaffolds were fabricated. The scaffolds were seeded with chondrocytes and cultured to evaluate the effect of elastic properties of polymer scaffolds for the differentiation and the ECM secretion of chondrocytes. Also, the chondrocytes-seeded constructs were implanted in nude mice subcutaneously to investigate their biocompatibility and cartilage formation. From the biochemical analyses, chondrogenic differentiation was sustained and enhanced significantly and chondral extracellular matrix was increased through mechanical stimulation of dynamic environment in the dynamic body systems. Histological analysis showed that implants of PLCL constructs formed mature and well-developed cartilaginous tissue, as evidenced by chondrocytes within lacunae. Consequently, the elastic PLCL scaffolds could be used to engineer cartilage in mechanically dynamic environments

Info:

Periodical:

Key Engineering Materials (Volumes 342-343)

Edited by:

Young-Ha Kim, Chong-Su Cho, Inn-Kyu Kang, Suk Young Kim and Oh Hyeong Kwon

Pages:

405-408

DOI:

10.4028/www.scientific.net/KEM.342-343.405

Citation:

Y. M. Jung et al., "Cartilage Tissue Engineering using a Elastic Poly (L-Lactide-co-ε-Caprolactone) Scaffold", Key Engineering Materials, Vols. 342-343, pp. 405-408, 2007

Online since:

July 2007

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Price:

$35.00

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