A Poly(β-Amino Ester) of Spermine and Poly(ethylene Glycol) Diacrylate as a Gene Carrier


Article Preview

Vectors are vital aspect of gene delivery system which decides the success of gene therapy. Efficient transfection with minimum or no toxicity, are two principal aims of any gene delivery system. In this our study, we rationally developed biodegradable water soluble poly(ßamino ester) (PAE) based on spermine (SPR) and poly (ethylene glycol) (PEG), by Michael-type addition reaction and further studied for its potential as a gene carrier. Confirmation of synthesized PAE was done by proton NMR spectroscopy. In gel retardation assay, the PAEs have shown good DNA binding ability over wide range of polyplexes. The addition of PEG over SPR resulted in a novel PAE with higher degree of safety and transfection efficiency as compared with polyethylenimine 25K (PEI) when studied in 293T human kidney carcinoma cells.



Key Engineering Materials (Volumes 342-343)

Edited by:

Young-Ha Kim, Chong-Su Cho, Inn-Kyu Kang, Suk Young Kim and Oh Hyeong Kwon




D. Jere et al., "A Poly(β-Amino Ester) of Spermine and Poly(ethylene Glycol) Diacrylate as a Gene Carrier", Key Engineering Materials, Vols. 342-343, pp. 425-428, 2007

Online since:

July 2007




[1] L. Huang, M.C. Hung, E. Wangner: Non-Viral vectors for Gene Therapy, Academic Press. New York.

[2] M.R. Park, K.O. Han, I.K. Han, M.H. Cho, J.W. Nah, Y.J. Choi, C. S. Cho : J. Cont. Rel. 105 (2005), pp.367-380.

[3] H. Eliyahu, A. Joseph, T. Azzam, Y. Barenholz, A.J. Domb : Biomaterials, 27 (2006), p.16361645.

[4] E.R. Lee, S.H. Cheng, et al.: Hum. Gene. Ther. 7 (1996), pp.1701-1717.

[5] A.J. Geall, I. Blagbrough,: Tetrahedron Lett., 39 (1998), pp.443-446.

[6] O. Boussif, B. Demeneix, et al.: Proc. Natl. Acad. Sci. USA. 92 (1995), pp.7297-301.

[7] B.D. Mather, K. Viswanathan, K.M. Miller, T.E. Long,: Prog. Poly. Sci., 31 (2006), pp.487-531.

[8] Y. Liu, D. Wu, Y. Ma, G. Tang, S. Wang, C. He, et al.: Chem. Comm. (2003), pp.2630-2631.

[9] A. Akinc, D.G. Anderson, D.M. Lynn and R. Langer, et al.: Bioconjugate Chem. 14 (2003), pp.979-988.

[10] D. Anderson, A. Akinc, N. Hossain, R. Langer : Mol. Ther., 11 (2005), pp.426-434.