Adhesion to Cells of Enzyme Conjugated Phospholipid Polymer Nanoparticles
We investigated the bioconjugation of proteins on polymer nanoparticles covered with bio-inert phosphorylcholine groups. Poly[2-methacryloyloxyethyl phosphorylcholine-co-n-butyl methacrylate-co-p-nitrophenyloxycarbonyl polyethyleneglycol methacrylate] was used as an emulsifier and a surface modifier to prepare the poly (L-lactic acid) nanoparticles by a solvent evaporation technique. The diameter and surface potential of the nanoparticles were approximately 260 nm and –5 mV, respectively. We considered that the polymer chain that transformed the conformation by change in pH may be used as a method of controlling the bioreaction. The enzyme activity in a different pH under the coexistence of poly (glutamic acid) (PGA) and the enzyme was measured. Therefore, the enzyme activity increased in pH 6 in PGA/enzyme mixture system compared with that in pH 7 while the activity was constant in the enzyme single conjugation regardless of the pH change.
Young-Ha Kim, Chong-Su Cho, Inn-Kyu Kang, Suk Young Kim and Oh Hyeong Kwon
T. Ito et al., "Adhesion to Cells of Enzyme Conjugated Phospholipid Polymer Nanoparticles", Key Engineering Materials, Vols. 342-343, pp. 785-788, 2007