Surface Modification by Grafting with Biocompatible 2-Methacryloyloxyethyl Phosphorylcholine for Microfluidic Devices

Abstract:

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Non-biofouling surfaces with polymer-based substrate were prepared for manufacturing microfluidic devices. It was done by constructing biocompatible poly(2-methacryloyloxyethyl phosphorylcholine(MPC)) brushes using surface-initiated graft polymerization method based on dithiocarbamate as photoiniferter. The density and length of the polymer chains were varied by changing the composition of the photoiniferter moiety in the base polymer (macrophotoiniferter) and the photoirradiation time, respectively. The molecular weight and thickness of the poly(MPC)- grafted chains were 320 kDa and 95±14 nm, respectively. Characterizations of the poly(MPC) modified surfaces were conducted by water contact angle, X-ray photoelectron spectroscopy, atomic force microscope. Protein adsorption resistance of these modified surfaces was then investigated by contacting with human plasma protein dissolved in phosphate buffered saline. These poly(MPC)-modified surfaces effectively reduced protein adsorption.

Info:

Periodical:

Key Engineering Materials (Volumes 342-343)

Edited by:

Young-Ha Kim, Chong-Su Cho, Inn-Kyu Kang, Suk Young Kim and Oh Hyeong Kwon

Pages:

789-792

DOI:

10.4028/www.scientific.net/KEM.342-343.789

Citation:

J. Sibarani et al., "Surface Modification by Grafting with Biocompatible 2-Methacryloyloxyethyl Phosphorylcholine for Microfluidic Devices", Key Engineering Materials, Vols. 342-343, pp. 789-792, 2007

Online since:

July 2007

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Price:

$35.00

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0 1. 0 3. 0 PEV10 PEV20 PEV30 PEV40.

0.

2.

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6.

8.

[1] 0.

[1] 2.

[1] 4.

[1] 6.

[1] 8.

[2] 0 Amount of adsorbed protein (μg/cm 2 ) Photoirradiation time (h) Figure 6. Total amount of adsorbed protein on macrophotoiniferter-coated surfaces (control surfaces) and on poly(MPC) brush surfaces on various chain density and irradiation time. Figure 5. 3D topography profile of poly(MPC) brush surfaces on different chain density for 3 hours photoirradiation time. PEV10-g-MPC3h (a), PEV20-g-MPC3h (b), PEV30-g-MPC3h (c), and PEV40-g-MPC3h with rms roughness of 27. 31 nm.

[9] 86 nm, 9. 83 nm, and 35. 79 for a, b c, and d respectively.

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