Study of Protein Adsorption on Biomaterial Surfaces by SPR

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The aim of this study was to set up a SPR sensor and to investigate protein adsorption onto biomaterial surface using it. Methods: The adsorption of human albumin (Alb), fibrinogen (Fib) and immunoglobulin G (IgG) onto polyurethane (PU) H50-50 and Au surface were measured using this SPR sensor. Results: The results of protein adsorption showed that the amount of Alb, Fib and IgG adsorbed on PU (H50-50) are smaller than Au, and on these two material surfaces, the amount sequence of adsorbed protein is: IgG> Fib > Alb. Both the Alb/Fib ratio and Alb/IgG ratio on PU (H50-50) surface are higher than Au. Conclusion: The antithrombogenicity of PU (H50-50) is better than Au.

Info:

Periodical:

Key Engineering Materials (Volumes 342-343)

Edited by:

Young-Ha Kim, Chong-Su Cho, Inn-Kyu Kang, Suk Young Kim and Oh Hyeong Kwon

Pages:

825-828

Citation:

J. He et al., "Study of Protein Adsorption on Biomaterial Surfaces by SPR", Key Engineering Materials, Vols. 342-343, pp. 825-828, 2007

Online since:

July 2007

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$38.00

[1] L. Wang, Z.L. Tao: Progress in Biochemistry and Biophysics, 1996, 23 (6): p.483.

[2] E. Stenberg, B. Persson, H. Roos, et al: Journal of Colloid and Interface Science, 1991, 143(2): p.513.

[3] R.J. Green, J. Davies, M.C. Davies, et al: Biomaterials, 1997, 18: p.405.

[4] R.J. Green, M.C. Davies, C.J. Roberts, et al: Biomaterials, 1999, 20: p.385.

[5] K.M. Hansson, T.P. Vikinge, M. Ranby, et al: Biosensors and Bioelectronics, 1999, 14(8-9): p.671.

[6] C.D. Xiao, S.F. Sui, Y.D. Gong, et al: Chinese Journal of Biomedical Engineering, 2000, 19(1): p.60.

[7] X.Y. Lü, Y. Huang, Z.M. Tang, et al: Chinese Journal of Biomedical Engineering 2003, 22(2): p.165 Fig. 4 The amount of the same protein adsorbed on two kinds of biomaterial surfaces Fig. 5 The resonance angle shift on Au and PU(H50-50).

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