Biological Evaluation of Sirolimus-Loaded PLGA Films


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Sirolimus-loaded poly (lactic acid-co-glycol acid) (PLGA) films were prepared by a casting method. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed to determine the optimal concentration of sirolimus for vascular smooth muscle cell (VSMC) inhibition effect. Based on the results of MTT, three samples of different weight percent loaded-sirolimus (2wt%, 6wt%, 15wt%) PLGA films were selected. The release of lactate dehydrogenase (LDH) was used to evaluate the immediate toxicity of the sirolimus-loaded PLGA films and it was shown that the three different concentrations of sirolimus-loaded films have low toxicity. Alamar blue results indicate that the sirolimus-loaded films have better antiproliferation effect than 316 stainless steel (SS). The higher the sirolimus concentration in the film is, the better is the antiproliferation effect. These films may be used for stent coating to inhibit in-stent restenosis induced by VSMC proliferation.



Key Engineering Materials (Volumes 342-343)

Edited by:

Young-Ha Kim, Chong-Su Cho, Inn-Kyu Kang, Suk Young Kim and Oh Hyeong Kwon




L. Ren et al., "Biological Evaluation of Sirolimus-Loaded PLGA Films", Key Engineering Materials, Vols. 342-343, pp. 837-840, 2007

Online since:

July 2007




[1] L. Barend,V. Hoeven and M.M. Nuno: International journal of Cardiology Vol. 9 (2005), P. 9.

[2] I. Ciro, M. Annalisa and C. Antonio: Therapy with Eluting Stents Vol. 13 (2003), P. 4.

[3] F. Versaci, A. Gaspardone and F. Tomai: IMPRESS study Vol. 40 (2002), P. (1935).

[4] M. Fujita, K. Mizuno and M. Ho: Am Heart Vol. 145 (2003), P. 16.

[5] S.N. Sehgal: Elsevier science Inc Vol. 35 (2003), P. 7.

[6] V. Ravindranath, N. Chandrasekhara: Toxicology Vol. 16 (1980), P. 259.

[7] A Racher. LDH Assay. In: Doyle A, Griffiths JB, editors. Cell and tissue culture, (1998), P. 71.

[8] S.A. Ahmed, Jr. RM. Gogal, J.E. Walsh: J Immunol Methods, Vol. 170 (1994), P. 211.

[9] M.F. Maitz, R.W.Y. Poon and X.Y. Liu: Biomaterials, Vol26 (2005) , P. 5465.

[10] B. Raz, M. Iten and Y. Grether-Buhler: Acta Trop Vol. 68 (1997), P. 139.

[11] Page B, Page M, Noel C: Int J Oncol, Vol. 3(1993), P. 473.

[12] M.A. P. faller, Q. Vu and M. Lancaster: J Clin Microbiol, Vol. 32 (1994), P. 1625 Fig. 5 Photos of DAPI staining after VSMC seeded on the three different concentrations of sirolimus-loaded PLGA films, and 316L stainless steel for 24h hours. ×200.