In Situ Investigation of Biomaterial Surfaces Using LDH and ELISA


Article Preview

Lactate dehydrogenase (LDH) and enzyme-linked immunosorbent assay (ELISA) have attracted much attention recently for the evaluation of blood compatibility of biomaterials due to their convenience and quantifiability. In this paper, the use of LDH and ELISA is described for in situ investigation of platelet behavior on biomaterial surfaces, including quantification of platelet adhesion and platelet activation, after suitable testing conditions have been established. The material samples investigated in these tests included low temperature isotropic carbon (LTIC), Ti-O films, and phosphorus- and aluminum-doped TiO2 films. The evaluation results show that the lowest platelet adhesion and activation are observed on phosphorus-doped TiO2 films while the highest platelet adhesion and activation are observed on LTIC. In addition, conventional platelet adhesion experiments were performed for comparison, and yielding similar evaluation results as LDH and ELISA. It is suggested that LDH and ELISA tests can be successfully applied to evaluate the blood compatibility of biomaterials and can show many advantages, such as quantification, reliability and objectivity, compared with conventional platelet adhesion test.



Key Engineering Materials (Volumes 342-343)

Edited by:

Young-Ha Kim, Chong-Su Cho, Inn-Kyu Kang, Suk Young Kim and Oh Hyeong Kwon




Z. Y. Shao et al., "In Situ Investigation of Biomaterial Surfaces Using LDH and ELISA", Key Engineering Materials, Vols. 342-343, pp. 841-844, 2007

Online since:

July 2007




[1] Andreas C. Eriksson and Per A. Whiss: Journal of Pharmacological and Toxicological Methods 52(2005), pp.356-365.

[2] Th. Groth, A. Podias and Y. Missirlis: Colloids and Surface B: Biointerfaces 3(1994), pp.24-249.

[3] Huang Nan, Yang Ping and Cheng Xuan: Biomaterials 19(1998), pp.771-776.

[4] P. Yang, N. Huang and Y.X. Leng: Biomaterials 24(2003), pp.2821-2829.

[5] Th. Groth, E.J. Campbell and K. Herrmann: Biomaterials 16(1995), pp.1009-1015.

[6] Joshua Downera, Joel R. Sevinskyb and Natalie G. Ahn: Journal of Theoretical Biology 240(2006), pp.464-474.

[7] Martine M. Fiore and Vijay V. Kakkar: Biochemical and Biophysical Research Communication 311(2003), pp.71-76.

[8] Piotr Radziwon and Barbara Boczkowska-Radziwon: Thrombosis Research 100(2000), pp.4-426.

[9] K. Gutensohn and A. Sputtek: Tranfus. Sci 17(1996), pp.489-492.

[10] Doug Lobner: Journal of Neuroscience Methods 96(2000), pp.147-152.

[11] J.Y. Chen, Y.X. Leng and X.B. Tian: Biomaterials 23(2002), pp.2545-2552.

[12] Teresa C. Diamantino, Elisabete Almeida and Amadeu M.V.M. Soares: Chemosphere 45 (2001), pp.553-560.


[13] Wataru Watanabe, Kenji Sudo and Sadanori Asawa: Journal of Virological Methods 51 (1995), pp.185-192.

[14] DM Maric, PF Meier and SK Estreicher: Biomaterials 50 (7) (1992), p.119.

[15] Th. Groth, A. Podias and Y. Missirlis: Colloids and Surface B: Biointerfaces 3(1994), pp.241-249.

[16] Cory C. Lalko, Elisabeth Deppe and Dan Ulatowski: Veterinary Immunology and Immunopathology 91(2003), pp.119-134.