Lactose Modified Chitosan Scaffold for Hepatocytes Culture


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The chitosan scaffold we prepared have a high porosity of about 90% with pore sizes from 50 to 200m. Lactose was conjugated onto the inner surface of the highly porous chitosan scaffold. It was used as substrate for rat hepatocytes culture. The cell attachment ratio was much higher than on monolayer membrane and non-modified porous scaffold. Metabolic activities of the cells were evaluated in terms of albumin secretion and urea synthesis. It was found that hepatocytes cultured on the modified scaffolds showed an increase in albumin secretion during the first 4 days and were more stable than that on non-modified scaffold. The results showed that the microstructure of porous scaffolds provides large surface for cells to adhere and facilitates nutrient and oxygen transportation. Such lactose modified scaffold has a potential application in bioartificial liver support system.



Key Engineering Materials (Volumes 353-358)

Edited by:

Yu Zhou, Shan-Tung Tu and Xishan Xie




J. L. Pan et al., "Lactose Modified Chitosan Scaffold for Hepatocytes Culture", Key Engineering Materials, Vols. 353-358, pp. 2147-2150, 2007

Online since:

September 2007




[1] IV Yannas, J Cell Biochem, 1994, 56(1): 88-91.

[2] JB Ma, HJ Wang, BL He, JT Chen, Biomaterials 2001, 22: 331-336.

[3] JK Suh, HW Matthew, Biomaterials, 2000, 21: 2589-2598.

[4] M Kawase, N Michibayashi, Y Nakashima et al , Biol Pharm Bull, 1997, 20: 708-710.

[5] K Mizuno, K Yamamura, K Yano, J Biomed Mater Res, 2003, 64: 177-181.

[6] Ruliang Li, Biochemistry Experiments. Wuhan University Press, 1998, 9-11.

[7] AC Puviani, C Ottolenghi, B Tassinari, Comp Biochem and Phys A. 1998, 120: 99-109.

[8] JL Li, JL Pan, LG Zhang, YT Yu, Biomaterials, 2003, 24: 2317-2322.

[9] YY Hsu, JD Gresser, DJ Trantolo et al, J Biomed Mater Res., 1997, 35(1): 107.

[10] Vivek Dixit, Artificial Organs, 1994, 18(5): 371-384.

[11] LD Bartolo, G. Catapano, CD Volpe, J. Biomater. Sci. Polymer Edn., 1999, 10(6): 641-655.

[12] Ba Zielinski, P ebischer, Biomaterials, 1994, 15: 1049-1056.

[13] TD Jiang, Chitin, China Environmental Science Press, 1999, 32-48.