The suspension bridge has more flexibility and repetitive vehicles produce stress cycles in members. Then fatigue of the member is accumulated with the daily traffic loadings. In order to evaluate the working condition of the Tsing Ma Bridge, the online monitoring health system has been installed in long suspension bridge. The location of the strain sensor is not exactly at the critical member locations. The hot spot stress analysis for critical members is necessary for accurate fatigue evaluation of the bridge. The global finite element analysis of the Tsing Ma Bridge under traffic loading is performed to determine the critical fatigue member locations. A detailed local finite element analysis for the welded connections is performed to determine the hot spot stress of critical fatigue location. As a case for study, the calculated stress concentration factor is combined with the nominal representative stress block cycle to obtain the representative hot spot stress range cycle block under traffic loading from online health monitoring system. The comparison result shows that the nominal stress approach cannot consider the most critical stress of the fatigue damage location and the hot spot stress approach is more appropriate for fatigue evaluation.