Effect of Acid Treated Hydroxyapatite on Osteoblast Maturation


Article Preview

Surfaces of sintered hydroxyapatite (HAp) were treated with four kinds of acids, hydrochloric acid (HCl), nitric acid (HNO3), sulfuric acid (H2SO4) and hydrogen peroxide (H2O2). On these acid-treated HAp surfaces, osteoblast-like MC3T3-E1 cells were cultured for 20 days. The matrix mineralization was observed in HCl-treated HAp and HNO3-treated HAp, but not in non-treated HAp, H2SO4-treated HAp and H2O2-treated HAp. Gene expressions of Bglap2 encoding osteocalcin and Akp2 encoding alkaline phosphatase were higher in HCl-treated HAp and HNO3-treated HAp than those in the others. These results indicate that not only hydrochloric acid but also nitric acid improves performance of HAp to mature osteoblast.



Key Engineering Materials (Volumes 361-363)

Main Theme:

Edited by:

Guy Daculsi and Pierre Layrolle




T. Takemura et al., "Effect of Acid Treated Hydroxyapatite on Osteoblast Maturation", Key Engineering Materials, Vols. 361-363, pp. 1029-1032, 2008

Online since:

November 2007




[1] W. Suchanek and M. Yoshimura: J. Mater. Res. Vol. 13 (1998), p.94.

[2] K.J.L. Burg, S. Porter and J.F. Kellam: Biomaterials Vol. 21 (2000), p.2347.

[3] U. Ripamonti: Biomaterials Vol. 17 (1996), p.31.

[4] G.A. Rodan: Bone Vol. 13 (1992), p. S3.

[5] T. Takemura, A. Monkawa, T. Ikoma, J. Tanaka and N. Hanagata: Proceedings of the sixth Asian BioCeramics Symposium (2006), p.167.

[6] T. Ikoma and A. Yamazaki: J. Mater. Sci. Lett. Vol. 18 (1999), p.1225.

[7] B. Messnaoui and T. Bounahmidi: Fluid Phase Equilibr. Vol. 244 (2006), p.117.

[8] S.L. Teitelbaum and F.P. Ross: Nat. Rev. Genet. Vol. 4 (2003), p.638.

[9] C.M. Kelly, R.M. Wilkins, S. Gitelis, C. Hartjen, J.T. Watson and P.T. Kim: Clin. Orthop. Relat. Res. Vol. 382 (2001), p.42.

[10] Á. Lazáry, B. Balla, J.P. Kósa, K. Bácsi, Z. Nagy, I. Takács, P.P. Varga, G. Speer and P. Lakatos: Biomaterials Vol. 28 (2007), p.393.

DOI: https://doi.org/10.1016/j.biomaterials.2006.09.002

[11] M. Navarro, S. del Valle, S. Martinez, S. Zeppetelli, L. Ambrosio, J.A. Planell and M.P. Ginebra: Biomaterials Vol. 25 (2004), p.4233.

DOI: https://doi.org/10.1016/j.biomaterials.2003.11.012