Effect of Ionic Exchange on Osteoblast Behaviour on Bioactive Tissue Engineering Substrates
In this work we examine the effect of the ionic exchange in cell behaviour of two apatite substrates with the same chemical composition but different microstructures (Coarse and Fine) by culturing cells with their extracts. A higher decrease in Ca and a higher increase in P concentrations were detected in the culture medium extracts from the finer apatite substrate in accordance with its higher specific surface area. A decrease in cell proliferation was observed when cells were cultured with the Fine extract in comparison with cells in contact with the Coarse extract or the control group. Although the higher ionic exchange, this could not explain the drastic decrease in cell proliferation observed on Fine apatite substrates, so a strong interaction with the underlying microstructure that modulates cell behaviour is also present.
Guy Daculsi and Pierre Layrolle
S. del Valle et al., "Effect of Ionic Exchange on Osteoblast Behaviour on Bioactive Tissue Engineering Substrates", Key Engineering Materials, Vols. 361-363, pp. 1051-1054, 2008