Stimulatory Effect on Osseous Repair of Zinc-Substituted Hydroxyapatite: Histological Study in Rabbit’s Tibia

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The study was carried out aiming the evaluation of the effect of hydroxyapatite (HA) and zinc-substituted hydroxyapatite (ZnHA) on osseous repair of rabbit’s tibia. For the study, 15 adult animals, weighing around 2.5 and 3.0 Kg, sourced by the Fluminense Federal University Animal Lab were acquired. Two perforations were created in each tibia and filled with cylinders (2x6 mm) of HA (group 1, right) or ZnHA (group 2, left). The animals were killed after 7, 14, and 28 days for evaluating the histological aspects of the interface site and bone repair. No sign of inflammatory reaction surrounding the cylinders area were observed, neither giant cells. Osteogenesis was evidently accelerated in all healing periods for the ZnHA group in regards to the HA group. Randomly dispersed areas of neoformed bone among wide areas of fibrous connective tissue was observed in the HA group. We conclude that both biomaterials are biocompatible, but zinc-containg HA enhanced and accelerated the osteogenesis in relation to HA.

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Periodical:

Key Engineering Materials (Volumes 361-363)

Main Theme:

Edited by:

Guy Daculsi and Pierre Layrolle

Pages:

1269-1272

DOI:

10.4028/www.scientific.net/KEM.361-363.1269

Citation:

M. Calasans-Maia et al., "Stimulatory Effect on Osseous Repair of Zinc-Substituted Hydroxyapatite: Histological Study in Rabbit’s Tibia", Key Engineering Materials, Vols. 361-363, pp. 1269-1272, 2008

Online since:

November 2007

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$35.00

[1] A. Uschida, N. Araki, Y. Shinto, H. Yoshikawa, E. Kurisaki, K. Ono. The use of calcium hydroxyapatite ceramic in bone human surgery. J Bone Joint Surg Br. 72: 298-302, (1990).

DOI: 10.1007/978-3-642-75879-9_24

[2] A. S. B. Moreira, M. T. Pastoreli, L. H. F. Damasceno, H. L. A. Defin. Estudo experimental da influência das dimensões dos grânulos de hidroxiapatita na integração óssea. Acta Ortop Bras. 11(4): 240-250, (2003).

DOI: 10.1590/s1413-78522003000400007

[3] A. Ito, K. Ojima, H. Naito, N. Ichinose, T. Tateishi. Preparation, solubility, and cytocompatibility of zinc-releasing calcium phosphate ceramics. J Biomed Mater Res. 50: 178-83, (2000).

DOI: 10.1002/(sici)1097-4636(200005)50:2<178::aid-jbm12>3.0.co;2-5

[4] M. Yamaguchi, H. Oishi, Y. Suketa. Stimulatory effect of zinc on bone formation in tissue culture. Biochem Pharm, 36(22): 4007-4012, (1987).

DOI: 10.1016/0006-2952(87)90471-0

[5] T.J. Webster, C. Ergun, R.H. Doremus, R. Bizios. Hydroxyapatite with substituted magnesium , zinc, cadmium, and yatrium II: mechanism of osteoblast adhesion. J Biomed Mater Res. Vol 59: 312-317, (2002).

DOI: 10.1002/jbm.1247

[6] T.J. Webster, E.A. Massa-Schlueter, J.L. Smith, E.B. Slamovich. Osteoblast response to hydroxyapatite doped with divalent and trivalent cations. Biomaterials. Vol 25: 2111-2121, (2004).

DOI: 10.1016/j.biomaterials.2003.09.001

[7] A. Ito, H. Kawamura, S. Miyakawa, P. Layrolle, R. Aomori, S. Tsutsumi. Preparation, solubility, and cytoompatibility of zinc-releasing calcium phosphate ceramics. Key Eng Mater. 192- 195, 199-202, (2001).

DOI: 10.4028/www.scientific.net/kem.192-195.199

[8] J. Brandão-Neto, V. Stefan, B.B. Mendonça, W. Bloise, A. V. B. Castro. The essential role of zinc in growth. Nutrition Research, 15(3): 335-358, 1995. A D B E C F Figure1. Photomicrographies showing the tissue remodeling around HA (A-C) and ZnHA (D-F) cylinders on 7, 14 and 28 days after surgery. Neoformed bone(arrow), connective tissue (�), biomaterial (*), cortical bone (CT). Magnification: 40X.

DOI: 10.1016/0271-5317(95)00003-8

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