Cellular Activity on Siloxane-Doped Poly(Lactic Acid)/Vaterite Composite Scaffolds

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Cellular activities of human mesenchymal stem cells (MSCs) and osteoblasts (HOBs) on a silicon-releasable scaffold, which is siloxane-doped poly(lactic acid) / vaterite composite coated with hydroxycarbonate apatite (SPV-H), were estimated using two types of media, with or without organic factors, dexamethasone (Dex) and β-glycerophosphate (β-GP). The culture tests using MSCs shows that the level of alkaline phosphatase (ALP) activity in the cells cultured on SPV-H increased for 21-day culturing in medium without Dex and β-GP. The proliferation of MSCs on SPV-H was significantly higher than that on a poly(lactic acid) / vaterite composite coated with hydroxyapatite (PV-H) at all time points. In the case of supplementing Dex and β-GP to the medium, the level of ALP activity in MSCs cultured on SPV-H was higher in comparison with that on PV-H at all time points. Scanning electron microscopy showed that there were some agglomerates in HOBs cultured on the SPV-H surface after 21-day culturing in the medium without the factors, while there are no agglomerates on PV-H. The agglomerates were regarded from laser Raman spectroscopy as bone nodules. This result implies stimulation to HOBs by silicon species in SPV-H. SPV-H is expected to be useful as the scaffold for bone tissue engineering.

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Periodical:

Key Engineering Materials (Volumes 361-363)

Main Theme:

Edited by:

Guy Daculsi and Pierre Layrolle

Pages:

399-402

DOI:

10.4028/www.scientific.net/KEM.361-363.399

Citation:

A. Obata and T. Kasuga, "Cellular Activity on Siloxane-Doped Poly(Lactic Acid)/Vaterite Composite Scaffolds", Key Engineering Materials, Vols. 361-363, pp. 399-402, 2008

Online since:

November 2007

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$35.00

[1] H. Maeda, T. Kasuga and L.L. Hench: Biomaterials Vol. 26 (2006), p.1216.

[2] I.D. Xynos, A.J. Edgar, L.D.K. Buttery, L.L. Hench and J.M. Polak: Biochem. Biophys. Res. Commun. Vol. 276 (2000), p.461.

[3] J.E. Gough, J.R. Jones and L.L. Hench: Biomaterials Vol. 25 (2004), p. (2039).

[4] J.R. Jones, O. Tsigkou, E.E. Coates, M.M. Stevens, J.M. Polak and L.L. Hench: Biomaterials Vol. 28 (2007), p.1653.

[5] D.M. Reffitt, N. Ogston, R. Jugdaohsingh, H.F.J. Cheung, B.A.J. Evans, R.P.H. Thompson, J.J. Powell and G.N. Hampson: Bone Vol. 32 (2003), p.127.

[6] N. Jaiswal, S.E. Haynesworth, A.I. Caplan and S.P. Bruder: J. Cell. Biochem. Vol. 64 (1997), p.295.

[7] H. Ohgushi and A.I. Caplan: J. Biomed. Mater. Res. Vol. 48 (1999), p.913.

[8] G. Ciapetti, L. Ambrosio, G. Marletta, N. Baldini and A. Giunti: Biomaterials Vol. 27 (2006), p.6150.

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