In Vitro Calcification of Silk Fibroin Hydrogel
Silk fibroin hydrogels were prepared and their potential to deposit calcium phosphates in vitro was observed. Pristine and lyophilized samples were tested in 1xSBF and 1.5xSBF. The results showed that silk fibroin hydrogels can induce calcium phosphate deposits both in the pristine and lyophilized form. However, the pristine silk fibroin hydrogel after calcification presented a fragile structure making it difficult to handle, while the lyophilized samples presented better resistance to handling. Calcium phosphates deposits were intense in samples submitted to tests in 1.5xSBF, however, few and isolated deposits were observed on samples submitted to tests in 1xSBF. The 3-D porous structure and the ability to deposit calcium phosphates, turn silk fibroin hydrogel a potential material suitable to use in biomimetic processes.
Guy Daculsi and Pierre Layrolle
G. M. Nogueira et al., "In Vitro Calcification of Silk Fibroin Hydrogel", Key Engineering Materials, Vols. 361-363, pp. 503-506, 2008