DNA-Coatings: Bioactive Properties and Effects on Osteoblast-Like Cells


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The aim of this study was to evaluate potential effects of DNA-coatings on calcium phosphate (CaP) nucleation from simulated body fluids (SBF) and subsequently the effects of DNA-coatings and SBF-immersed DNA coatings on the behavior of osteoblast-like cells. DNAcoatings demonstrated to enhance the nucleation and deposition of CaP from SBF compared to titanium controls. The behavior of osteoblast-like cells was affected on SBF-immersed DNAcoatings, showing an increased deposition of the extracellular matrix protein osteocalcin compared to titanium controls. These results indicate bone-bonding capacity of DNA-coatings, which needs to be confirmed using future animal experiments.



Key Engineering Materials (Volumes 361-363)

Main Theme:

Edited by:

Guy Daculsi and Pierre Layrolle




J. J.J.P. van den Beucken et al., "DNA-Coatings: Bioactive Properties and Effects on Osteoblast-Like Cells", Key Engineering Materials, Vols. 361-363, pp. 605-608, 2008

Online since:

November 2007




[1] Ducheyne P, Bianco, P, Radin, S, Scherpers, E. Bioactive Materials: Mechanisms and Bioengineering Considerations. 1992. Leiderdorp, the Netherlands, Reed Healthcare Communications. Bone-bonding biomaterials. Ducheyne, P., Kokubo, T., and van Blitterswijk, C. A.

DOI: https://doi.org/10.1002/jbm.820271112

[2] Kokubo T, Ito, S, Huang, ZT, Hayashi, T, Sakka, S, Kitsugi, T, Yamamuro, T. Ca, P-rich layer formed on high-strength bioactive glass- ceramic A-W. J Biomed Mater Res 1990; 24: 331-43.

DOI: https://doi.org/10.1002/jbm.820240306

[3] Liu Q, Ding, J, Mante, FK, Wunder, SL, Baran, GR. The role of surface functional groups in calcium phosphate nucleation on titanium foil: a self-assembled monolayer technique. Biomaterials 2002; 23: 3103.

DOI: https://doi.org/10.1016/s0142-9612(02)00050-9

[11] [4] Tanahashi M, Matsuda, T. Surface functional group dependence on apatite formation on self-assembled monolayers in a simulated body fluid. J Biomed Mater Res 1997; 34: 305-15.

DOI: https://doi.org/10.1002/(sici)1097-4636(19970305)34:3<305::aid-jbm5>3.0.co;2-o

[5] van den Beucken JJ, Vos, MR, Thune, PC, Hayakawa, T, Fukushima, T, Okahata, Y, Walboomers, XF, Sommerdijk, NA, Nolte, RJ, Jansen, JA. Fabrication, characterization, and biological assessment of multilayered DNA-coatings for biomaterial purposes. Biomaterials 2006; 27: 691-701.

DOI: https://doi.org/10.1016/j.biomaterials.2005.06.015

[6] van den Beucken JJ, Walboomers, XF, Vos, MR, Sommerdijk, NA, Nolte, RJ, Jansen, JA. Cyto- and histocompatibility of multilayered DNA-coatings on titanium. J Biomed Mater Res A 2006; 77A: 202-211.

DOI: https://doi.org/10.1002/jbm.a.30583

[7] van den Beucken JJJP, Walboomers, XF, Vos, MRJ, Sommerdijk, NJAM, Nolte, RJM, Jansen, JA. Macrophage behavior on multilayered DNA-coatings in vitro . J Biomed Mater Res A.

DOI: https://doi.org/10.1002/jbm.a.30971

[8] van den Beucken JJ, Walboomers, XF, Boerman, OC, Vos, MR, Sommerdijk, NA, Hayakawa, T, Fukushima, T, Okahata, Y, Nolte, RJ, Jansen, JA. Functionalization of multilayered DNA-coatings with bone morphogenetic protein 2. J Control Release 2006; 113: 63-72.

DOI: https://doi.org/10.1016/j.jconrel.2006.03.016

[9] van den Beucken JJ, Walboomers, XF, Nillesen, ST, Vos, MR, Sommerdijk, NA, van Kuppevelt, TH, Nolte, RJ, Jansen, JA. In vitro and in vivo effects of deoxyribonucleic acid-based coatings funtionalized with vascular endothelial growth factor. Tissue Eng 2007; 13: 711-20.

DOI: https://doi.org/10.1089/ten.2006.0303

[10] Kokubo T, Kushitani, H, Sakka, S, Kitsugi, T, Yamamuro, T. Solutions able to reproduce in vivo surfacestructure changes in bioactive glass-ceramic A-W. J Biomed Mater Res 1990; 24: 721-34.

DOI: https://doi.org/10.1002/jbm.820240607

[11] Kokubo T, Takadama, H. How useful is SBF in predicting in vivo bone bioactivity? Biomaterials 2006; 27: 2907-15.

DOI: https://doi.org/10.1016/j.biomaterials.2006.01.017

[12] van den Dolder J, Bancroft, GN, Sikavitsas, VI, Spauwen, PH, Jansen, JA, Mikos, AG. Flow perfusion culture of marrow stromal osteoblasts in titanium fiber mesh. J Biomed Mater Res A 2003; 64: 235-41.

DOI: https://doi.org/10.1002/jbm.a.10365