In Vitro Dissolution Behavior of Custom Made CaP Scaffolds for Bone Tissue Engineering


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The degradation rate of custom made calcium phosphate scaffolds, designed for bone tissue engineering applications, influences the healing process of critical size bone defects. An optimal degradation rate exists at which the neo-formed bone replaces the CaP (calcium phosphate) scaffold [1]. Consequently investigating the complex degradation behavior (dissolution, reprecipitation, osteoclast activity) of custom made CaP structures gains interest. In this work different in vitro dissolution experiments were performed to study the degradation behavior of 4 by composition different calcium phosphates. Ideally these experiments should have a predictive power regarding the in vivo degradation behavior. In vitro dissolution tests still lack standardization. Therefore this study focuses on the influence of two dissolution constraints: (i) the material’s macrostructure (porous - dense), (ii) the regenerated fluid flow (bath shaking - perfusion). From 4 different CaP compositions porous structures and as a reference dense disks were produced, using the same starting powder and heat treatment. To compare the different dissolution tests, all data was normalized to the CaP surface area. Results show that besides the structural appearances of the CaP structures, also the design of the dissolution test influences the in vitro dissolution behavior. Moreover there is a need to take the morphology of the dissolved material into account. The CaP perfusion tests show dissolution dynamics that resemble the in vivo reality more closely than the shaking bath experiments.



Key Engineering Materials (Volumes 361-363)

Main Theme:

Edited by:

Guy Daculsi and Pierre Layrolle




S. Impens et al., "In Vitro Dissolution Behavior of Custom Made CaP Scaffolds for Bone Tissue Engineering", Key Engineering Materials, Vols. 361-363, pp. 7-10, 2008

Online since:

November 2007




[1] X-W Wang (2003), Journal of Medical and Biological Engineering, 23, 3 pp.159-164.

[2] M. Mastrogiacomo et al. (2006), Biomaterials, 26, 17, pp.3230-3237.

[3] T.L. Arinzeh et al. (2005), Biomaterials, 26, 17 pp.3631-3638.

[4] K.H. Hing et al. (2007), The Spine Journal, In Press, Corrected Proof.

[5] M. -H.Y. Cheung et al. (2007), Composites Part B: Engineering, 38, 3 pp.291-300.

[6] S. Yamada et al. (1997) Biomaterials, 18, 15 pp.1037-1041.

[7] J. Luyten et al. (2003), Advanced Engineering Materials, 5 , 10 pp.715-718.

[8] T. Van Cleynenbreugel et al. (2006), Med Biol Eng Comput; 44, 7 pp.517-25.

[9] P. Ducheyne (1993).