Protein Adsorption of Highly (001) Oriented Hydroxyapatite Coating Using Rf-Plasma Spraying

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The protein adsorption behavior was investigated for highly (001) oriented hydroxypatatite coatings (HACs). Highly (001) oriented (HO-) HACs and HAC with low orientation (LO-HAC) were prepared on titanium (Ti) substrates through a radio-frequency thermal plasma spraying method. Sintered HA pellets (S-HA) was also prepared as a control. The solution of 22 'g/100 'l PBS of the fluorescein-isothiocyanate (FITC) labeled bovine serum albumin (FITC-BSA), immunoglobulin G (FITC-IgG) and cytochrom c (FITC-CCC) was separately dropped on the surface of HACs and was incubated for 30 min. After the incubation, such HACs were washed with the PBS additionally supplemented with 125mM NaCl and observed using a fluorescence microscope. Fluorescence microscopic examination indicates that FITC labeled proteins somewhat adsorbed on the HACs, while proteins adsorbed little on S-HA surface. In particular, it can be seen that FITC-CCC adsorbed more prominently on the HO-HAC.

Info:

Periodical:

Key Engineering Materials (Volumes 361-363)

Main Theme:

Edited by:

Guy Daculsi and Pierre Layrolle

Pages:

705-708

DOI:

10.4028/www.scientific.net/KEM.361-363.705

Citation:

M. Inagaki et al., "Protein Adsorption of Highly (001) Oriented Hydroxyapatite Coating Using Rf-Plasma Spraying", Key Engineering Materials, Vols. 361-363, pp. 705-708, 2008

Online since:

November 2007

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$35.00

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