Calcium Phosphate Coated Rapid Prototyped Porous Titanium Scaffolds
Porous Titanium Scaffolds were produced by using a rapid prototyping technique. These scaffolds were either coated or not with a calcium phosphate coating via an eletrodeposition method. Rat bone marrow mesenchymal stem cells were cultured on the scaffolds at a density of 106 cells/scaffold for a period of 3 days. Cell proliferation was measured by using the Alamar Blue assay. The scaffolds were observed by SEM and polarized light microscopy. Constructs were then implanted subcutaneously for 4 weeks in syngenic rats. Cells proliferated well after seeding. After subcutaneous implantation, histology and SEM revealed the presence of uniform coatings as well as Ca and P deposits in the non-coated scaffolds suggesting mineralization.
Guy Daculsi and Pierre Layrolle
M. A. Lopez-Heredia et al., "Calcium Phosphate Coated Rapid Prototyped Porous Titanium Scaffolds", Key Engineering Materials, Vols. 361-363, pp. 907-910, 2008