Chemical Detoxification of AflatoxinB1 in Rice by Several Solutions through Fluorescence Spectral Experiment
AflatoxinB1 was extracted from rice contaminated artificially with Aspergillus flavus by using methanol-water (50:50 v/v). As for experiment group, sodium chloride was added into the extracted solution of AfB1, the excitation wavelength of 365 nm under the fluorescence mode, and the emission spectrum peak at 440nm were observed. To study the detoxification of AflatoxinB1 in several alkaline solutions, the solution of alkaline with difference concentration was added into the solution of AfB1 respectively. The results showed that all of three alkaline solutions could decrease the 440nm emission spectrum peak of solution of AfB1 in varying degrees, the effect to decrease emission spectrum peak of AfB1 was very obvious in sodium hypochlorite (NaClO), and sodium hydroxide (NaOH) took second place, whereas in ammonia (NH3·H2O), the effect was the weakest among three alkaline solutions. Moreover, the effects were different in the same alkaline solutions with different concentration, as hydrochloric acid (HCl) in low concentration was added to this complex AfB1 alkaline solutions, detoxification efficacy of AfB1 were found to be obviously increased. It was concluded that the detoxification of AflatoxinB1 may be affected by alkaline solutions according to their concentration and be degraded greatly by acid addition.
Guo Fan JIN, Wing Bun LEE, Chi Fai CHEUNG and Suet TO
W. L. Chen et al., "Chemical Detoxification of AflatoxinB1 in Rice by Several Solutions through Fluorescence Spectral Experiment", Key Engineering Materials, Vols. 364-366, pp. 1032-1036, 2008