Microscopic Three-Dimensional Imaging Theory Based on RIKES


Article Preview

A novel nonlinear confocal microscopic imaging system based on Raman induced Kerr effect spectroscopy (RIKES) is presented in this paper. The three-dimensional (3-D) microscopic imaging theory is derived with the Fourier imaging theory and nonlinear optical principle. The impact of RIKES on the spatial resolution and imaging properties of confocal microscopic imaging system has been analyzed in detail by the imaging theory. It’s proved that the RIKES nonlinear microscopic imaging system can effectively improve the imaging contrast and provide more characteristic information on Raman spectrum and optical nonlinear Kerr effect, thus greatly improving the imaging quality of confocal microscopic imaging system. It’s shown that the spatial resolution of RIKES confocal microscopic imaging system is higher than that of two-photon confocal microscopic imaging system.



Key Engineering Materials (Volumes 364-366)

Edited by:

Guo Fan JIN, Wing Bun LEE, Chi Fai CHEUNG and Suet TO




L. N. Guo et al., "Microscopic Three-Dimensional Imaging Theory Based on RIKES", Key Engineering Materials, Vols. 364-366, pp. 1089-1094, 2008

Online since:

December 2007




[1] G. Cox and C. J. R. Sheppard, Microsc. Res. Techniq. (2003), 18.

[2] P. T. C. So, Opt. Exp., Vol. 3 (1998), 312.

[3] B. Masters and P. So: Opt. Exp., Vol. 8 (2001), 2.

[4] Z. L. Tang, C. P. Yang, H. J. Pei, et al: Sci. in Chin., Ser. A, Vol. 45 (2002), 1468.

[5] Z. L. Tang, D. Xing, and S. H. Liu: Sci. Chin., Ser. G, Vol. 47 (2004), 8.

[6] T. Boulesteix, E. Beaurepaire, M. P. Sauviat, et al: Opt. Lett., Vol. 29 (2004), (2031).

[7] A. Volkmer, J. Phys. D, Vol. 38 (2005), R59.

[8] J. H. Yuan, F. R. Xiao, C. F. Cheng, et al, Colloid Surface A, Vol. 257-258 (2005), 525.

[9] C. Egami, N. Kobayashi, Y. Kawata, et al, Opt. Lett., Vol. 31 (2006), 784.

[10] L. Sacconi, I. M. Tolic´-Nørrelykke, R. Antolini, et al: J. Biomed. Opt., Vol. 10 (2005), p.014002.

[11] A. Zumbusch, G. R. Holtom, and X. S. Xie: Phys. Rev. Lett. Vol . 82 (1999), p.4142.

[12] T. Yasui, K. Minoshima, E. Abraham, et al: Appl. Opt. Vol. 41 (2002), p.5191.

[13] E. O. Potma, W. P. de Boeij, and D. A. Wiersma: Biophys. J. Vol. 80 (2001), p.3019.

[14] G. Giraud, J. Karolin, and K. Wynne: Biophy. Vol. 85 (2003), p. (1903).

[15] D. Heiman, R. W. Hellwarth, M. D. Levenson, et al: Phys. Rev. Lett. Vol. 36 (1976), p.189.

[16] P. S. Bhatia and J. W. Keto: Phys. Rev. A Vol. 59 (1999), p.4045.

[17] G. L. Eesley: Coherent Raman spectroscopy (Pergamon Press, America 1981).

[18] S. X. Qian and G. M. Wang: Nonlinear Optics (Fudan Univer. Press, China 2001).