In this study, the author uses a novel nanofluid synthesis system to fabricate a TiO2 nanofluid. The improvement of the proposed nanofluid synthesis system focuses mainly on the pressure control system, coolant circulation system, parameter control system and the machine dimension of the original submerged arc nanofluid synthesis system. This helps to achieve an experimental machine with a fabrication condition to produce more stable and finer TiO2 nanofluids with a particle size of good reproducibility. Experiment is proceeded towards 15 sets of TiO2 nanofluid fabricated by the proposed system are tested under the experimental conditions of 250 V, 6 A of peak current, 2μs of discharge pause off time and 15 days of settling time. The experimental results show that the average Zeta potential of TiO2 nanofluids are -54.2 mv, and the difference between the data and the average value of each set is less than 7%. Furthermore, the average particle size is 45.3nm, and the difference between the data and the average value of each set is less than 6%. The fabricated TiO2 particles have an Anatase structure, and in the aspect of roundness measurement, the produced TiO2 has a good roundness of 0.3 nm. Experiment proves that the roundness of the fabricated TiO2 nanoparticles are much better than those fabricated by aerosol methods. Also, the fabricated nanofluid has a high suspension stability.