A translucent amido modified microemulsion was synthesized by the adding of amido silane coupling agent. Transmission electron microscope (TEM), Fourier Transform Infrared Spectra (FT-IR), Photon Correlation Spectroscopy (PCS) were used to characterize the structures and properties of amido microemulsion, respectively. IR spectroscopy confirmed that amido group was successfully introduced into the polysiloxane microemulsion. TEM images indicated that the granules agglomerated heavily when the coupling agent was in a larger content. The effects of PH values, amount of coupling agent on the size and the configuration of the amido microemulsion were investigated. The results indicated that the properties of amido microemulsion were stable when the microemulsion was under weak acid condition (pH=3-7). When the amount of coupling agent increased, the particles size of microemulsion increased and the particle size distribution became narrow.