In order to study the damage evolution law for layered fiber reinforced concrete subjected to flexural fatigue, the flexural fatigue tests were carried out on both layered steel fiber reinforced concrete(LSFRC) and layered hybrid fiber reinforced concrete(LHFRC) beams of which the type of steel fiber was uniform on the same concrete mix. At the same time the flexural fatigue tests with original concrete(OC) were carried out. Based on the experiments, both flexural fatigue life and damage characteristic of LSFRC LHFRC and OC were compared and analyzed. The results indicated that the fatigue life of LHFRC was a little larger than that of LSFRC under the stress level, which was more than an order of magnitude of OC. And the fatigue distortion of LSFRC, LHFRC and OC were similar. They all followed three-phase law. However, the proportion of every phase was different, which proved that layered steel fibers and polypropylene fibers could effectively restrain the degradation of concrete.