Reconstruction of the Stomach with Smooth Muscle Cell Seeded Constructs in Rat Model


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We have developed a cell-seeded 3-dimension constructs tissue regeneration and used it successfully to repair defects of bone and cartilage. However, improvements of mechanical strength were demanded for clinical usage of reconstruction of gastrointestinal tracts. Rat model was used to evaluate whether PLGA constructs seeded with smooth muscle cells (SMC) could withstand suturing and anastomotic strength. Macrscopic, histological, and immunohistochemical assessment were performed to evaluate regeneration of stomach up to 14 days. SMC-seeded constructs show sufficient strength for suturing, no dehiscence, and better biocompatibility for clinical use, although further examination will be necessary to create a functional digestive tract.



Key Engineering Materials (Volumes 467-469)

Edited by:

Dehuai Zeng




Y. X. Zheng et al., "Reconstruction of the Stomach with Smooth Muscle Cell Seeded Constructs in Rat Model", Key Engineering Materials, Vols. 467-469, pp. 1876-1880, 2011

Online since:

February 2011




[1] R. Langer, J.P. Vacanti: Tissue engineering. Science Vol. 260 (1993), pp.920-926.

[2] Y. Zheng, J. Ringe and Z. Liang: Osteogenic potential of human periosteum-derived progenitor cells in PLGA scaffold using allogeneic serum. J Zhejiang Univ SCIENCE B Vol. 7 (2006), pp.817-824.


[3] Y. Nakase, A. Hagiwara and T. Nakamura: Tissue engineering of small intestinal tissue using collagen sponge scaffolds seeded with smooth muscle cells. Tissue Eng Vol. 12 (2006), p.403.


[4] J. Ringe, C. Kap, and G. Rr Burmester: Stem cells for regenerative medicine: advances in the engineering of tissues and organs. Naturwissenschaften Vol. 89 (2002), pp.338-351.


[5] F.G. Rocha, E.E. Whang: Intestinal tissue engineering: from regenerative medicine to model systems. J Surg Res Vol. 120 (2004), p.320.

[6] M. Sittinger, D.M. Hutmacher and M.V. Risbud. Current strategies for cell delivery in cartilage and bone regeneration. Curr Opin Biotechnol Vol. 15 (2004), pp.411-418.