Investigations on Vitro Behaviour of Ceramic Coatings Deposited by High Velocity Suspension Flame Spraying
Bioceramics used as coatings show different biocompatibility and bioactive behavior in relation to their chemical and morphological behavior. Bioactive ceramics such as β-tricalcium phosphate (β-TCP) promote and enhance biological fixation. Stable coatings require an optimum between resorption rate, flexural strength and adhesive strength of the coating. Therefore new bioceramic coating materials that ensure the balance between loss of substances and osteointegration need to be designed and investigated. By modifying the high velocity suspension flame spraying (HVSFS) process parameters, five coatings with different materials were obtained. The in vitro cytotoxicity was determined by the microculture tetrazolium (WST) assay after 24, 48 and 72 h. Cells were grown on the materials for 3, 7, 14, and 21 days and counted. Cell morphology, cell attachment, and cell spreading were investigated using fluorescence microscopy and raster scanning electron microscopy. All substrates supported sufficient cellular growth for 19 days and showed no cytotoxicity. On each material an identical cell colonisation of well communicating, polygonal, vital cells was verified.
Eyup Sabri Kayali, Gultekin Goller and Ipek Akin
A. Bernstein et al., "Investigations on Vitro Behaviour of Ceramic Coatings Deposited by High Velocity Suspension Flame Spraying", Key Engineering Materials, Vols. 493-494, pp. 530-534, 2012