Investigations on Vitro Behaviour of Ceramic Coatings Deposited by High Velocity Suspension Flame Spraying

Abstract:

Article Preview

Bioceramics used as coatings show different biocompatibility and bioactive behavior in relation to their chemical and morphological behavior. Bioactive ceramics such as β-tricalcium phosphate (β-TCP) promote and enhance biological fixation. Stable coatings require an optimum between resorption rate, flexural strength and adhesive strength of the coating. Therefore new bioceramic coating materials that ensure the balance between loss of substances and osteointegration need to be designed and investigated. By modifying the high velocity suspension flame spraying (HVSFS) process parameters, five coatings with different materials were obtained. The in vitro cytotoxicity was determined by the microculture tetrazolium (WST) assay after 24, 48 and 72 h. Cells were grown on the materials for 3, 7, 14, and 21 days and counted. Cell morphology, cell attachment, and cell spreading were investigated using fluorescence microscopy and raster scanning electron microscopy. All substrates supported sufficient cellular growth for 19 days and showed no cytotoxicity. On each material an identical cell colonisation of well communicating, polygonal, vital cells was verified.

Info:

Periodical:

Key Engineering Materials (Volumes 493-494)

Main Theme:

Edited by:

Eyup Sabri Kayali, Gultekin Goller and Ipek Akin

Pages:

530-534

DOI:

10.4028/www.scientific.net/KEM.493-494.530

Citation:

A. Bernstein et al., "Investigations on Vitro Behaviour of Ceramic Coatings Deposited by High Velocity Suspension Flame Spraying", Key Engineering Materials, Vols. 493-494, pp. 530-534, 2012

Online since:

October 2011

Export:

Price:

$35.00

[1] Legeros RZ. Calcium phosphate-based osteoinductive materials. Chem Rev. 2008 Nov; 108(11): 4742-53.

DOI: 10.1021/cr800427g

[2] Döbelin N, Luginbühl R, Bohner M. Synthetic calcium phosphate ceramics for treatment of bone fractures. Chimia (Aarau). 2010; 64(10): 723-9.

DOI: 10.2533/chimia.2010.723

[3] Overgaard S. Calcium phosphate coating for fixation of bone implants. Acta Orthop Scand 2000; 71: 1–74.

[4] Kay JF. Bioactive surface coatings for hard tissue biomaterials. In: Yamamuro T, Hench LL, Wilsch J, editors. CRC Handbook of Bioactive Ceramics 1990; 2: 111–112.

[5] Liu X, Chu PK, Ding C. Surface modification of titanium titanium alloys, and related materials for biomedical applications. Mater Sci Eng R. 2004; 47: 49–121.

DOI: 10.1016/j.mser.2004.11.001

[6] Altomare L, Bellucci D, Bolelli G, Bonferroni B, Cannillo V, De Nardo L, Gadow R, Killinger A, Lusvarghi L, Sola A, Stiegler N. Microstructure and in vitro behaviour of 45S5 bioglass coatings deposited by high velocity suspension flame spraying (HVSFS). J Mater Sci Mater Med. 2011 May; 22(5): 1303-19. Epub 2011 Apr 3.

DOI: 10.1007/s10856-011-4307-6

[7] Bolelli G, Cannillo V, Gadow R, Killinger A, Lusvarghi L, Rauch J. Microstructural and in vitro characterisation of high-velocity suspension flame sprayed (HVSFS) bioactive glass coatings. J Eur Ceram Soc. 2009; 29: 2249–57.

DOI: 10.1016/j.jeurceramsoc.2009.01.032

[8] Bolelli G, Cannillo V, Gadow R, Killinger A, Lusvarghi L, Rauch J, Romagnoli M. Effect of the suspension composition on the microstructural properties of high velocity suspension flame sprayed (HVSFS) Al2O3 coatings. Surf Coat Technol. 2010; 204: 1163–79.

DOI: 10.1016/j.surfcoat.2009.10.045

[9] Bernstein A, Nöbel D, Mayr HO, Berger G, Gildenhaar R, Brandt J. Histological and histomorphometric investigations on bone integration of rapidly resorbable calcium phosphate ceramics. J Biomed Mater Res B Appl Biomater. 2008 Feb; 84(2): 452-62.

DOI: 10.1002/jbm.b.30891

[10] Porter AE, Patel N, Skepper JN, Best SM, Bonfield W. Comparison of in vivo dissolution processes in hydroxyapatite and silicon-substituted hydroxyapatite bioceramics. Biomaterials 2003; 24: 4609–4620.

DOI: 10.1016/s0142-9612(03)00355-7

[11] Hench LL, Wilson J. Introduction. Hench LL, Wilson J, editors. An Introduction to Bioceramics. Singapore: World Scientific; 1993. p.1–24.

DOI: 10.1142/9789814317351_0001

[12] Bächle M, Kohal RJ. A systematic review of the influence of different titanium surfaces on proliferation, differentiation and protein synthesis of osteoblast-like MG63 cells. Clin Oral Implants Res. 2004 Dec; 15(6): 683-92.

DOI: 10.1111/j.1600-0501.2004.01054.x

[13] Ducheyne P, Qiu Q. Bioactive ceramics: The effect of surface reactivity on bone formation and bone cell function. Biomaterials 1999; 20: 2287–2303.

DOI: 10.1016/s0142-9612(99)00181-7

[14] Ohtsuki C, Kamitakahara M, Miyazaki T. Bioactive ceramic-based materials with designed reactivity for bone tissue regeneration. J R Soc Interface. 2009 Jun 6; 6 Suppl 3: S349-60.

DOI: 10.1098/rsif.2008.0419.focus

In order to see related information, you need to Login.