Preparation and Characterization of Polylactide-co-glycolide/Carbonate Apatite (PLGA/CHA) Composite Scaffolds

Abstract:

Article Preview

Both natural and synthetic materials have been utilized to provide three dimensional scaffold environments ideal for bone repair. The biomechanical and biocompatibility characteristics of these scaffolds play a vital role in successful tissue engineering constructs. Polymer/carbonate apatite (CHA) composites have shown to improve cell adhesion and proliferation on the scaffold as well as increase elastic modulus, toughness and strength. The aim of this study is to prepare CHA- polylactic-co-glycolide (PLGA) composites in the form of microsphere, scaffold and disc and evaluate their physico-chemical properties, mechanical properties and in vitro bioactivity. 3-D porous cylindrical composite scaffolds were prepared using PLGA/CHA composites with varying PLGA/CHA ratios (30:70 and 50:50). The CHA was prepared by hydrolysis method and characterized using x-ray diffraction (XRD) and Fourier Transform Infrared spectroscopy (FTIR). The physico-chemical and mechanical properties of the composite scaffolds were evaluated using scanning electron microscopy (SEM), micro-computed tomography (μCT), XRD, FTIR, and thermogravimetry (TGA). Flexural strength was determined using Instron. In vitro bioactivity was determined by the formation of apatite on composite disc surfaces after immersion in simulated body fluid (SBF). SEM and μCT analyses showed high porosity and interconnectivity between microspheres in the composite scaffolds. In vitro bioactivity was observed by the development of an apatite layer on the surfaces of the composite scaffolds after immersion in simulated body fluid. The mechanical strength of the scaffolds was to be dependent on the PLGA-CHA ratio. The elastic modulus, toughness and strength values obtained for the composites were similar to those of reported bone substituted materials. Results from this study provided information on the fabrication of PLGA-CHA scaffolds and their properties that may be useful for their potential application in bone repair and as scaffolds in tissue engineering for bone regeneration.

Info:

Periodical:

Key Engineering Materials (Volumes 493-494)

Main Theme:

Edited by:

Eyup Sabri Kayali, Gultekin Goller and Ipek Akin

Pages:

572-576

DOI:

10.4028/www.scientific.net/KEM.493-494.572

Citation:

H. E. Stone et al., "Preparation and Characterization of Polylactide-co-glycolide/Carbonate Apatite (PLGA/CHA) Composite Scaffolds", Key Engineering Materials, Vols. 493-494, pp. 572-576, 2012

Online since:

October 2011

Export:

Price:

$35.00

[1] Lu HH (2005 ). Compositional effects on the formation of a calcium phosphate layer and the response of osteoblast-like cells on polymer-bioactive glass composites. Biomaterials 26: 11.

DOI: 10.1016/j.biomaterials.2005.04.005

[2] Rose FO, R. (2002). Bone tissue engineering: hope vs hype. Biochemical and Biophysical Research Communications 292: 1-7.

[3] Lu HH (2002).

[4] Kauth H (2005). Examination of the dynamic mechanical properties of tissue engineering scaffolds. Material Research Society 844: 5.

[5] LeGeros RZ, LeGeros JP, Trautz OR, Shirra WP (1971) Conversion of monetite, CaHPO4 to apatite: Effect of carbonate on the crystallinity and the morphology of the apatite crystallites. Adv X-ray Anal 14: 57-66.

DOI: 10.1126/science.155.3768.1409

[6] LeGeros RZ (1991). Calcium Phosphates in Oral Biology and Medicine. Monographs in Oral Sciences Vol. 15. Myers H (Ed). Karger: Basel.

[7] Laurencin C. T ESea (1998). A Highly Porous 3-dimensional polyphosphazene polymer matrix for skeletal tissue regeneration. Journal of Biomedical Materials Research 30: 5.

[8] Kokubo TO, T. Kushitani, H. Kotani, S. Yamamuro, T. (1991). Apatite formation on the surface of Ceravital-type glass-ceramic in the body. Journal of Biomedical Materials Research 25: 7.

DOI: 10.1002/jbm.820251105

[9] Armento ID, M. Fortunati, E. Mattioli, S. Kenny, JM. (2010). Biodegradable polymer matrix nanocomposites for tissue engineering: A review. Polymer Degradation and Stability 95: 2126-2146.

DOI: 10.1016/j.polymdegradstab.2010.06.007

[10] Rezwan K, Chen QZ, Blaker JJ, Boccaccini AR (2006). Biodegradable and bioactive porous polymer/inorganic composite scaffolds for bone tissue engineering. Biomaterials 27: 3413-3431.

DOI: 10.1016/j.biomaterials.2006.01.039

[11] Seal BL OT, Panitch A. (2001). Polymeric biomaterials for tissue and organ regeneration. Material Science Engineering 34: 147-230.

[12] Stefely J, Schultz D, Leach C, Duan D (2010). Biocompatible compounds for sustained release pharmaceutical drug delivery systems. Patent No.: US 7, 687, 054 B2 Date of Patent: Mar. 30, (2010).

In order to see related information, you need to Login.