Regulating Protein Adsorption onto Hydroxyapatite: Amino Acid Treatment

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Hydroxyapatite (HA) has been widely used as bone grafts due to its chemical and structural similarities to the mineral phase of hard tissues. Applying the combination of osteogenic proteins with HA materials can accelerate bone regeneration in defective areas. The aim of the study was investigating the treatment of HA particles with different amino acids such as serine (Ser), asparagine (Asn), aspartic acid (Asp) and arginine (Arg) to enhance the adsorption ability of HA carrier for delivering therapeutic proteins in body. Results: The crystallinity of HA reduced when amino acids were added during HA preparation. Depending on the types of amino acid, the specific surface area of the amino acid-functionalized HA particles varied from 105 to 149 m2/g. Bovine serum albumin (BSA) and lysozyme were used as model proteins for adsorption study. The protein adsorption onto the surface of amino acid-functionalized HA depended on the polarities of HA particles, whereby positively charged Arg-HA had higher affinity towards BSA (0.269 mg/m2) compared to lysozyme (0.133 mg/m2). Alternatively, the binding affinity of lysozyme (0.2 mg/m2) onto the negatively charged Asp-HA was higher compared to BSA (0.129 mg/m2). The amino acids functionalized-HA particles that had higher proteins adsorption demonstrated a lower protein release rate.

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Periodical:

Key Engineering Materials (Volumes 493-494)

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Edited by:

Eyup Sabri Kayali, Gültekin Göller and Ipek Akin

Pages:

666-671

Citation:

W.H. Lee et al., "Regulating Protein Adsorption onto Hydroxyapatite: Amino Acid Treatment", Key Engineering Materials, Vols. 493-494, pp. 666-671, 2012

Online since:

October 2011

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$38.00

[1] S. Bodhak, S. Bose, and A. Bandyopadhyay, Role of surface charge and wettability on early stage mineralization and bone cell-materials interactions of polarized hydroxyapatite, Acta Biomater, 5 (2009) 2178-2188.

DOI: https://doi.org/10.1016/j.actbio.2009.02.023

[2] L.C. Xu and C.A. Siedlecki, Effects of surface wettability and contact time on protein adhesion to biomaterial surfaces, Biomaterials, 28 (2007) 3273-83.

DOI: https://doi.org/10.1016/j.biomaterials.2007.03.032

[3] X. Qu, et al., The effect of oxygen plasma pretreatment and incubation in modified simulated body fluids on the formation of bone-like apatite on poly(lactide-co-glycolide) (70/30), Biomaterials, 28 (2007) 9-18.

DOI: https://doi.org/10.1016/j.biomaterials.2006.08.024

[4] S. Kidoaki and T. Matsuda, Mechanistic aspects of protein/material interactions probed by atomic force microscopy, Colloid Surface B, 23 (2002) 153-163.

DOI: https://doi.org/10.1016/s0927-7765(01)00232-6

[5] M.H. Uddin, et al., Apatite Containing Aspartic Acid for Selective Protein Loading, J Dent Res, 89 (2010) 488-492.

DOI: https://doi.org/10.1177/0022034509357309

[6] K.S. Jack, T.G. Vizcarra, and M. Trau, Characterization and surface properties of amino-acid-modified, carbonate-containing hydroxyapatite particles, Langmuir, 23 (2007) 12233-12242.

DOI: https://doi.org/10.1021/la701848c

[7] B. Palazzo, et al., Amino acid synergetic effect on structure, morphology and surface properties of biomimetic apatite nanocrystals, Acta Biomater, 5 (2009) 1241-1252.

DOI: https://doi.org/10.1016/j.actbio.2008.10.024

[8] G. Yin, et al., Impacts of the surface charge property on protein adsorption on hydroxyapatite, Chem Eng J, 87 (2002) 181-186.

[9] S. Dasgupta, A. Bandyopadhyay, and S. Bose, Reverse micelle-mediated synthesis of calcium phosphate nanocarriers for controlled release of bovine serum albumin, Acta Biomater, 5 (2009) 3112-3121.

DOI: https://doi.org/10.1016/j.actbio.2009.04.031

[10] T. Matsumoto, et al., Hydroxyapatite particles as a controlled release carrier of protein, Biomaterials, 25 (2004) 3807-12.

[11] R.Z. Legeros, Apatites in Biological-Systems, Progress in Crystal Growth and Characterization of Materials, 4 (1981) 1-45.

[12] Y.L. Chen, et al., Conformational changes of fibrinogen adsorption onto hydroxyapatite and titanium oxide nanoparticles, J Colloid Interf Sci, 214 (1999) 38-45.