Hydroxyapatite Scaffolds Produced by Hydrothermal Deposition of Monetite on Polyurethane Sponges Substrates


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Hydroxyapatite scaffolds have been being produced by a wide range of processes. The optimun material to be used as bone graft has to be partially resorbable, with resorption rates similar to new bone formation ones. The samples must have porosity compatible with tissue ingrowth. Hydroxyapatite and tricalcium phosphate ceramics are good choices for designing such materials. In the present study, polymeric sponges were coated with hydroxyapatite and sintered. The method consists of coating polyurethane sponges substrates in an aqueous solution rich in phosphate (PO4)3- and calcium (Ca)2+ ions. The solution is composed by 0.5M Ca(OH)2, 0.3M H3PO4 and 1M CH3CHCO2HOH (lactic acid) at pH of 3.7. The sponges were immersed in a beaker with the solution and heated up to 80°C to precipitate monetite on the sponge. Continuous and adherent coatings were formed on the surface of sponges interconections. These coatings were characterised by X-ray diffractometry and the only identified phase was monetite. The substrates were converted to hydroxyapatite in an alkali solution.The total conversion from monetite to hydroxyapatite was confirmed by XRD analyses. The struts were heat treated in order to eliminate the organic sponge and sinter the scaffolds. After sintering, hydroxyapatite and tricalcium phosphate were identified on the struts. Optical microscopy revealed the morphology of the struts, while scanning electron microscopy (SEM) showed the precipitates morphology. The method showed to be efficient in the production of porous scaffolds.



Key Engineering Materials (Volumes 493-494)

Main Theme:

Edited by:

Eyup Sabri Kayali, Gültekin Göller and Ipek Akin




F. D. Mishima et al., "Hydroxyapatite Scaffolds Produced by Hydrothermal Deposition of Monetite on Polyurethane Sponges Substrates", Key Engineering Materials, Vols. 493-494, pp. 820-825, 2012

Online since:

October 2011




[1] K. So, S. Fujibayashi, M. Neo, Y. Anan, T. Ogawa, T. Kokubo, T. Nakamura, Accelerated degradation and improved bone-bonding ability of hydroxyapatite ceramics by addition glass, Biomaterials. 27 (2006) 4738-4744.

DOI: https://doi.org/10.1016/j.biomaterials.2006.05.020

[2] Q. Liu, L. Cen, S. Yin, L. Chen, G. Liu, J. Chang, L. Cui, A comparative study of proliferation and osteogenic differentiation of adipose-derived stem cells on akermanite and β-TCP ceramics, Biomaterials. 29 (2008) 4792-4799.

DOI: https://doi.org/10.1016/j.biomaterials.2008.08.039

[3] C. Wu, Y. Zhang, Y. Zhu, T. Friis, Y. Xiao, Structure-property relationships of silk-modified mesoporous bioglass scaffolds, Biomaterials. 31 (2010) 3429-3438.

DOI: https://doi.org/10.1016/j.biomaterials.2010.01.061

[4] E.Y. Kawashi, C.A. Bertran, R.R. Reis, O.L. Alves, Biocerâmicas: tendências e perspectivas de uma área interdisciplinar. Química Nova, 23-4 (2000) 518-522.

DOI: https://doi.org/10.1590/s0100-40422000000400015

[5] L.L. Hench, Bioceramics, J Am Ceram Soc. 81 (1998) 1705-1728.

[6] R.Z. Le Geros, Properties of osteoconductive biomaterials: calcium phosphates, Clin Orthop Rel Res. 395 (2002) 81-98.

[7] S. Langstaff, M. Sayer, T.J.N. Smith, S.M. Pugh, Resorbable bioceramics based on stabilized calcium phosphates. Part II: evaluation of biological response, Biomaterials. 22 (2001) 135-150.

DOI: https://doi.org/10.1016/s0142-9612(00)00139-3

[8] M.H. Prado da Silva, J.H.C. Lima, G.A. Soares, C.N. Elias, M.C. de Andrade, S.M. Best, I.R. Gibson, Transformation of monetite to hydroxyapatite in bioactive coatings on titanium, Surface and Coatings Technology. 137 (2001) 270-273.

DOI: https://doi.org/10.1016/s0257-8972(00)01125-7

[9] L. Hench, J.M. Polak, Third Generation Biomedical Materials science. 295 (2002) 1014-1017.

[10] Y.L. Liu, J. Schoenars, T.K. Groot, J.R. Wijn, E. Schepers, Bone Healing in Porous Implants; A Histological and Histometrical Comparative Study on sheep, Journal of Materials: Materials in Medicine. 11 (2000) 711-717.

[11] S. Padilla, R. Garci Carrodeguas, M. Vallet - Regi, Hydroxyapatite suspensions as precursors of pieces obtained by gelcasting method, Journal of the European Ceramic Society. 24 (2004) 2223–2232.

DOI: https://doi.org/10.1016/s0955-2219(03)00629-0

[12] I.O.R. Gibson, S. Ke, S.M. Best, W. Bonfield, Effect of powder characteristics on the sinterability of hydroxyapatite powders, Journal of Materials Science: Materials in Medicine. 12 (2001) 163-171.

[13] N.O. Patel, I.O.R. Gibson, S. Ke, S.M. Best, W. Bonfield, Calcining influence on the powder properties of hydroxyapatite, Chemistry and Materials Science. 12 (2004) n. 2.