Effect of Flow Rate of Medium in Radial-Flow Bioreactor on the Differentiation of Osteoblasts in Tissue-Engineered Bone Reconstructed Using an Apatite-Fiber Scaffold and Rat Bone Marrow Cells

Abstract:

Article Preview

Tissue engineering has been studied as a novel therapeutic technology which replaces organ transplantation. Tissue engineering consists of three factors “scaffolds”, “cells” and “growth factors”, and regenerates defecting tissue using them. We have successfully developed porous apatite-fiber scaffolds (AFSs) which have three-dimensional (3D) inter-connected pores using single-crystal apatite fibers and carbon beads; subsequently, we have clarified that the AFSs have an excellent bioactivity on the basis of both in vitro and in vivo evaluations. In addition, we have reconstructed the 3D tissue-engineered bone through 3D-cell culture of mesenchymal stem cells derived from rat bone marrow (RBMC) using the AFS settled into the radial-flow bioreactor (RFB). Aim in the present work is to examine the effect of flow rate of medium in the RFB on the differentiation of osteoblasts in tissue-engineered bone reconstructed using an AFS and RBMC. The flow rates were set to 1.3 and 6.3 cm3∙min-1; tissue-engineered bones reconstructed by the two flow rates are defined as “bone#1” and “bone#2”, respectively. The ALP activity and OC amount normalized for DNA content of bone#2 were higher than those of bone#1. These results indicate that the faster flow rate may promote the differentiation into osteoblast. Thus, the physical irritation to cells, such as flow rates, may be effective for reconstruction of tissue-engineered bone.

Info:

Periodical:

Key Engineering Materials (Volumes 493-494)

Main Theme:

Edited by:

Eyup Sabri Kayali, Gültekin Göller and Ipek Akin

Pages:

878-883

Citation:

M. Miura et al., "Effect of Flow Rate of Medium in Radial-Flow Bioreactor on the Differentiation of Osteoblasts in Tissue-Engineered Bone Reconstructed Using an Apatite-Fiber Scaffold and Rat Bone Marrow Cells", Key Engineering Materials, Vols. 493-494, pp. 878-883, 2012

Online since:

October 2011

Export:

Price:

$41.00

[1] M. Aizawa, H. Shinoda, H. Uchida, I. Okada, T. J. Fujimi, N. Kanazawa, H. Morisue, M. Matsumoto and Y. Toyama, Characterization of Porous b-tricalcium Phosphate Films Formed on Alumina Ceramics by Spray-pyrolysis Technique and Their in vitro Evaluations Using Osteoblasts, Phosphorus Res. Bull. 17 (2004).

DOI: https://doi.org/10.2109/jcersj.113.245

[2] H. Morisue, M. Matsumoto, K. Chiba, H. Matsumoto, Y. Toyama, M. Aizawa, N. Kanzawa, T. J. Fujimi, H. Uchida, I. Okada, A novel hydroxyapatite fiber mesh as a carrier for recombinant human bone morphogenetic protein-2 enhances bone union in rat posterolateral fusion model. Spine. 31 (2006).

DOI: https://doi.org/10.1097/01.brs.0000217679.46489.1b

[3] J. Fukasawa, Y. Nakada, H. Maehashi, T. Matsuura and M. Aizawa, Reconstruction of tissue-engineered bone through combination of an apatite-fiber scaffold, a radial-flow bioreactor and rat bone marrow cells, The 3rd International Congress on Ceramics (ICC3), Osaka International Convention Center, Osaka, 14-18 November (2010).

DOI: https://doi.org/10.4028/www.scientific.net/kem.529-530.397

[4] T. Iwahori, T. Matsuura, H. Maehashi, K. Sugo, M. Saito, M. Hosokawa, K. Chiba, T. Masaki, H. Aizaki, K. Ohkawa and T. Suzuki, CYP3A4 inducible model for in vitro analysis of human drug metabolism using a bioartificial liver, Hepatology. 37 (2002).

DOI: https://doi.org/10.1053/jhep.2003.50094

[5] M. Aizawa, F. S. Howell, K. Itatani, Y. Yokogawa, K. Nishizawa, M. Toriyama and T. Kameyama, Fabrication of Porous Ceramics with Well-controlled Open Pores by Sintering of Fibrous Hydroxyapatite Particles, J. Ceram. Soc. Jpn. 108 (2000) 249-253.

DOI: https://doi.org/10.2109/jcersj.108.1255_249

[6] M. Aizawa, A. E. Porter, S. M. Best, and W. Bonfield, Ultrastructural Observation of Single-crystal Apatite Fibres, Biomaterials. 26 (2005) 3427-3433.

DOI: https://doi.org/10.1016/j.biomaterials.2004.09.044

[7] Michelle R. Kreke, William R. Huckle, Aaron S. Goldstein, Fluid flow stimulates expression of osteopontin and bone sialoprotein by bone marrow stromal cells in a temporally dependent manner, Bone. 36 (2005) 1047–1055.

DOI: https://doi.org/10.1016/j.bone.2005.03.008