Biological Evaluation of a Novel Tissue Engineering Scaffold of Layered Double Hydroxides (LDHs)


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Bone Tissue Engineering (BTE) composed of three main parts: scaffold, cells and signaling factors. Several materials and composites are suggested as a scaffold for BTE. Biocompatibility is one of the most important property of a BTE scaffold. In this work synthesis of a novel nanocomposite including layered double hydroxides (LDH) and gelatin is carried out and its biological properties were studied. The co-precipitation (pH=11) method was used to prepare the LDH powder, using calcium nitrate, Magesium nitrate and aluminum nitrate salts as starting materials. The resulted precipitates were dried. X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) analyses were used to characterize the synthesized powders. The results demonstrated the presence of nanocrystals of Ca-LDH and Mg-LDH as Hexagonal and Layered Morphology. The obtained powders were composed to gelatin via solvent casting method then freez dried. The scaffold was prepared via membrane lamination method from the resulted layers that linked together with gelatin as binder. In order to investigate the scaffold cytotoxicity MTT assay was done with a osteosarcoma cell line. No toxic response was observed in specimens. As a major result, it was demonstrated that the specimen showed a significant cellular response. Then osteosarcoma cells were cultured for 7-day and 14-day extract of powders. The composites osteoconductivity was investigate with cells alkaline phosphatase extraction. The results demonstrated that the Ca-LDH/gelatin composite scaffold has a good potential for bone tissue engineering applications and Mg-LDH specimen has a better osteconductivity.



Key Engineering Materials (Volumes 493-494)

Main Theme:

Edited by:

Eyup Sabri Kayali, Gultekin Goller and Ipek Akin






F. Fayyazbakhsh et al., "Biological Evaluation of a Novel Tissue Engineering Scaffold of Layered Double Hydroxides (LDHs)", Key Engineering Materials, Vols. 493-494, pp. 902-908, 2012

Online since:

October 2011




[1] Jin-Ho Choy, Soo-Jin Choi, Jae-Min Oh, Taeun Park, Clay minerals and layered double hydroxides for novel biological applications, Applied Clay Science 36 (2007) 122–132.

DOI: 10.1016/j.clay.2006.07.007

[2] M. S. Azimi, Z. Tahmasebi, A. Darvish, S. S. Shafiei, and M. Solati-hashjin, Ca/Al Layered Double Hydroxides Hydrothermally Modified for Biomaterials Applications, European Cells and Materials Journal, Vol. 19. Suppl. 1, p.19 (2010).

[3] A.B. Burzlaff, S. Kasper, C. Jackisch, B. -O. Scheper, T, Flow Cytometry: Interesting Tool for Studying Binding Behavior of DNA on Inorganic Layered Double Hydroxide (LDH. CYTOMETRY PART A, 2004. 62: pp.65-69.

DOI: 10.1002/cyto.a.20085

[4] H. -W. Olfs , L.O. Torres-Dorante a R. Eckelt , H. Kosslick , Comparison of different synthesis routes for Mg–Al layered double hydroxides (LDH): Characterization of the structural phases and anion exchange properties, Applied Clay Science 43 (2009).

DOI: 10.1016/j.clay.2008.10.009

[5] Katharina Ladewig , Marcus Niebert , Zhi Ping Xu , Peter P. Gray , Gao Qing (Max) Lu, Controlled preparation of layered double hydroxide nanoparticles and their application as gene delivery vehicles, Applied Clay Science 48 (2010) 280–289.

DOI: 10.1016/j.clay.2009.11.032

[6] John P. Fisher, A.G.M., Joseph D. Bronzino, Tissue Engineering2007: CRC Press.

[7] Duan. X., and Evans. D.G. (Editors), Layered double hydroxides: structure and bondings, Springer, 200.

[8] Khan. A.I., and O'Hare. D., Intercalation chemistry of layered double hydroxides: recent developments and applications, journal of materials chemistry, V. 12, 3191–3198, (2002).

DOI: 10.1039/b204076j

[9] Cheng, K., et al., In vitro behavior of osteoblast-like cells on fluoridated hydroxyapatite coatings. Biomaterials, 2005. 26(32): pp.6288-6295.

DOI: 10.1016/j.biomaterials.2005.03.041

[10] Ferraz, M., et al., Flow cytometry analysis of the effects of pre-immersion on the biocompatibility of glass-reinforced hydroxyapatite plasma-sprayed coatings. Biomaterials, 2000. 21(8): pp.813-820.

DOI: 10.1016/s0142-9612(99)00249-5

[11] RZ. LeGeros, Properties of osteoconductive biomaterials: calcium phosphates. Clin Orthopedics and Related Researchs, 2002. 395: pp.81-95.

DOI: 10.1097/00003086-200202000-00009

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