Synthesis and Properties of Molecular Beacon DNA Probe Bearing Novel Silylated Pyrene Derivative
A novel stem-loop structured fluorescent oligoDNA probe (molecular beacon probe) bearing a silylated pyrene derivative at C-5 position of deoxyuridine has been synthesized. The fluorescently labeled modified nucleoside has been incorporated into two consecutive positions in the stem segment of the DNA using an automated DNA synthesizer. The resulting modified DNA exhibited an excimer fluorescent signal upon binding to the fully matched complementary DNA strand. The excimer emission was, however, effectively quenched while it stays alone or it hybridizes to a single base mismatched complementary target.
Osamu Hanaizumi, Masafumi Unno and Kenta Miura
M. G. Uddin et al., "Synthesis and Properties of Molecular Beacon DNA Probe Bearing Novel Silylated Pyrene Derivative", Key Engineering Materials, Vol. 497, pp. 47-50, 2012