Biomimic Enamel Remineralization by Hybridization Calcium- and Phosphate-Loaded Liposomes with Amelogenin-Inspired Peptide
We here report a novel biomimetic mineralization strategy for enamel remineralization by intergration of calcium phosphate loaded and thermally triggered liposomes and a self-assembly amelogenin-inspired peptide. Firstly, calcium and phosphate loaded temperature sensitive liposomes were synthesized by Interdigitation-fusion method with 1,2-bis(palmitoyl)-sn-glycero-3-phosphor- choline (DPPC) and 1,2-bis(myristoyl)-sn-glycero- 3-phosphocholine (DMPC) at mass ratio of 9:1 . The liposomes were stable at room temperature, but slowly released calcium and phosphate ions if heated to 37 °C. Secondly, a novel polyanion amelogenin-inspired oligropeptide (Gln-Pro-Ala)4-Thr-Lys-Arg-Glu-Glu-Val-Asp ) was synthesized by standard solid-phase. Lastly, the mixture of peptide and liposomes solution was exposed to enamel surface at 37 °C. The results showed oriented enamel-like hydroxylapatite evenly deposited on enamel surface.
Wei Pan and Jianghong Gong
J. J. Luo et al., "Biomimic Enamel Remineralization by Hybridization Calcium- and Phosphate-Loaded Liposomes with Amelogenin-Inspired Peptide", Key Engineering Materials, Vols. 512-515, pp. 1727-1730, 2012