Fabrication of Spatially-Patterned Cells Using Selective Adhesion on Pre-Structured Fine Particles


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The application of a neuron network to a bio/micro-sensor has potential as a drug screening device. In this study, micro-structures of surface-modified particles were applied to a scaffold for selective cell adhesion and growth. Silica particles were covered with a specific protein (fibronectin) or multi-walled carbon nanotubes (CNT) by electrostatic adsorption or transfer printing, respectively. They formed spatial patterns in a line-and-space structure tens of micrometers wide on a glass substrate. This paper investigates the effect of the coated material on the selectivity and adhesiveness of PC12 phenochromocytoma cells. An incubation process causes PC12 cells to autonomously align with selective adhesion on the micro-structures of both particles. The cells are minimally adhered to the glass surface around the particles. The structure of the fibronectin-coated particles enables a straight and uniform alignment of adhered cells, while that of bare silica particles causes randomly distributed cells. It was also found that the structure of CNT-adsorbed particles enhances cell adhesiveness to grow pseudopods of adhered cells.



Key Engineering Materials (Volumes 523-524)

Edited by:

Tojiro Aoyama, Hideki Aoyama, Atsushi Matsubara, Hayato Yoshioka and Libo Zhou




A. Kaneko et al., "Fabrication of Spatially-Patterned Cells Using Selective Adhesion on Pre-Structured Fine Particles", Key Engineering Materials, Vols. 523-524, pp. 615-620, 2012

Online since:

November 2012




[1] Y. Kanamori, A. Kaneko, N. Moronuki, T. Kubo, Self-Assembly of Fine Particles on Patterned Wettability in Dip Coating and Its Scale Extension with Contact Printing, Journal of Advanced Mechanical Design, Systems, and Manufacturing, 2, 4 (2008).

DOI: https://doi.org/10.1299/jamdsm.2.783

[2] A. Kaneko, I. Takeda, Y. Tanaka, N. Moronuki, M. Nishio, Micro-patterning of Biomaterials using Self-assembled Micro-spheres, Proc. 6th International Conference on Micro Manufacturing, (2011) 599–603.

[3] I. Takeda, A. Kaneko, Y. Tanaka, N. Moronuki, Selective Cell-adhesion on Micro-structured Fine Particles, Key Engineering Materials, (to be published).

DOI: https://doi.org/10.4028/www.scientific.net/kem.516.130

[4] Y. Ito, Surface micropatterning to regulate cell function, Biomaterials, 20 (1999) 2333–2342.

[5] N. Aoki, T Akasaka, F. Watari, A. Yokoyama, Carbon Nanotubes as Scaffolds for Cell Culture and Effect on Cellular Functions, Dental materials journal, 26(2), (2007) 178–185.

DOI: https://doi.org/10.4012/dmj.26.178

[6] M. Alkan, G. Tekin, H. Namli, FTIR and zeta potential measurements of sepiolite treated with some organosilanes, Microporous and Mesoporous Materials, 84 (2005) 75–83.

DOI: https://doi.org/10.1016/j.micromeso.2005.05.016

[7] A. Bouafsoun, S. Helali, S. Mebarek, C. Zeiller, Electrical probing of endothelial cell behaviour on a fibronectin/polystyrene/thiol/gold electrode by Faradaic electrochemical impedance spectroscopy (EIS), Bioelectrochemistry, 70 (2007) 401–407.

DOI: https://doi.org/10.1016/j.bioelechem.2006.05.012

[8] K. Yoshino, A. Kaneko, Y. Tanaka, and N. Moronuki, Fabrication of Micro-cantilever Structure Using Self-assembled Particles, Proc. 6th International Conference on Leading Edge Manufacturing in 21st Century, (2011), 3287-1–6.

DOI: https://doi.org/10.1299/jsmelem.2011.6._3287-1_

[9] A. Baba, F. Sato, N. Fukuda, H. Ushijima, K. Yase, Micro/nanopatterning of single-walled carbon nanotube–organic semiconductor composites, Nanotechnology, 20 (2009) 085301-1–6.

DOI: https://doi.org/10.1088/0957-4484/20/8/085301

[10] T. Goto, A. Kaneko, Y. Tanaka, and N. Moronuki, CNT Adsorption and Micro-patterning of Spherical Silica Particles, Proc. 6th International Conference on Leading Edge Manufacturing in 21st Century, (2011), 3286-1–6.

DOI: https://doi.org/10.1299/jsmelem.2011.6._3286-1_

[11] A. Bédur, L. Vaysse, E. Flahaut, F. Seichepine, I. Loubinoux, C. Vieu, Multi-scale engineering for neuronal cell growth and differentiation, Microelectronic Engineering, 88 (2011) 1668–1671.

DOI: https://doi.org/10.1016/j.mee.2010.12.049