Microstructuring of Culture Media and its Effect on Cell Adhesion


Article Preview

Control of adhesion force between cells and medical materials is important for artificial tissue such as tooth, bone and joint. There are many trials to control the adhesion by modifying the surface of culture media. One of these is roughness control of culture media. In this study, the cells cultured on microstructured surface were pushed by a cantilever on a micromanipulator. The deflection of cantilever was measured to calculate the force, and the crucial force when cells were detached from media was quantified as cell adhesion force. Microstructured surface was fabricated from poly-dimethylsiloxane (PDMS). The structure was replicated from Si substrate that was etched through a mask of self-assembled particles. The profile of the mold is cone array of which pitches is same with mask particle's diameter 1 or 5 μm. Thus, the media has regular pits which correspond to the pillar. The effect of pitch and height of the structures on the adhesion force were made clear.



Key Engineering Materials (Volumes 523-524)

Edited by:

Tojiro Aoyama, Hideki Aoyama, Atsushi Matsubara, Hayato Yoshioka and Libo Zhou




Y. Tanaka et al., "Microstructuring of Culture Media and its Effect on Cell Adhesion", Key Engineering Materials, Vols. 523-524, pp. 674-679, 2012

Online since:

November 2012




[1] S. Britland, P. Clark, P. Connolly, G. Moores, Micropatterned substratum adhesiveness: A model for morphogenetic cues controlling cell behavior, Exp. Cell Res. 198 (1992) 124-129.

DOI: https://doi.org/10.1016/0014-4827(92)90157-4

[2] M. Mrksich, C.S. Chen, Y. Xia, L.E. Dike, D.E. Ingber, G.M. Whitesides, Controlling cell attachment on contoured surfaces with self-assembled monolayers of alkanethiolates on gold, Proc. Natl. Acad. Sci. U.S.A. 93 (1996) 10775-10778.

DOI: https://doi.org/10.1073/pnas.93.20.10775

[3] S.B. Carter, Happtptaxis and the Mechanism of Cell Motility, Nature, 21 (1967) 256-260.

[4] H. Kobayashi, N. Moronuki, A. Kaneko, Self-assembly of Fine Particles Applied to the Production of Antireflective Surface, Int. J. Precis. Eng. Man. 9 (2008) 25-29.

[5] M. Nishio, A. Kaneko, N. Moronuki, Complex Assembly of Fine Particles by Repeated Dip-coating Process, 3rd International Conf. of Asian Society for Precision Engineering and Nanotechnology (2009).

[6] C. Yan, A. Yersin, R. Afrin, H. Sekiguchi, A. Ikai, Single molecular dynamic interactions between glycophorin A and lectin as probed by atomic force microscopy, Biophys. Chem. 144 (2009) 72-77.

DOI: https://doi.org/10.1016/j.bpc.2009.06.009

[7] Y. Shen, M. Nakajima, S. Kojima, M. Homma, T. Fukuda, Study of the time effect on the strength of cell-cell adhesion force by a novel nano-picker, Biochem. Biopys. Res. Commun. 409 (2011) 160-165.

DOI: https://doi.org/10.1016/j.bbrc.2011.04.019